A fast click-slow release strategy towards the HPLC-free synthesis of RNA

E. Agustin; P. N. Asare Okai; I. Khan; M. R. Miller; R. Wang; J. Sheng; M. Royzen

doi:10.1039/c5cc05392g

A fast click-slow release strategy towards the HPLC-free synthesis of RNA

E. Agustin
, P. N. Asare Okai
, I. Khan
, M. R. Miller
, R. Wang
, J. Sheng
, M. Royzen

Chemistry

University at Albany

SUNY Albany

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

A general strategy for purification of oligonucleotides synthesized by solid phase synthesis is described. It is based on a recently developed concept involving a bio-orthogonal inverse electron demand Diels-Alder reaction between trans-cyclooctene and tetrazine, termed 'click-to-release'. The strategy has been applied towards the synthesis and purification of a model hairpin RNA strand, as well as a 34 nt long aptamer.

Original language	English
Pages (from-to)	1405-1408
Number of pages	4
Journal	Chemical Communications
Volume	52
Issue number	7
DOIs	https://doi.org/10.1039/c5cc05392g
State	Published - Jan 25 2016

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title = "A fast click-slow release strategy towards the HPLC-free synthesis of RNA",

abstract = "A general strategy for purification of oligonucleotides synthesized by solid phase synthesis is described. It is based on a recently developed concept involving a bio-orthogonal inverse electron demand Diels-Alder reaction between trans-cyclooctene and tetrazine, termed 'click-to-release'. The strategy has been applied towards the synthesis and purification of a model hairpin RNA strand, as well as a 34 nt long aptamer.",

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AU - Agustin, E.

AU - Asare Okai, P. N.

AU - Khan, I.

AU - Miller, M. R.

AU - Wang, R.

AU - Sheng, J.

AU - Royzen, M.

PY - 2016/1/25

Y1 - 2016/1/25

N2 - A general strategy for purification of oligonucleotides synthesized by solid phase synthesis is described. It is based on a recently developed concept involving a bio-orthogonal inverse electron demand Diels-Alder reaction between trans-cyclooctene and tetrazine, termed 'click-to-release'. The strategy has been applied towards the synthesis and purification of a model hairpin RNA strand, as well as a 34 nt long aptamer.

AB - A general strategy for purification of oligonucleotides synthesized by solid phase synthesis is described. It is based on a recently developed concept involving a bio-orthogonal inverse electron demand Diels-Alder reaction between trans-cyclooctene and tetrazine, termed 'click-to-release'. The strategy has been applied towards the synthesis and purification of a model hairpin RNA strand, as well as a 34 nt long aptamer.

UR - https://www.scopus.com/pages/publications/84954462135

U2 - 10.1039/c5cc05392g

DO - 10.1039/c5cc05392g

M3 - Article

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SN - 1359-7345

VL - 52

SP - 1405

EP - 1408

JO - Chemical Communications

JF - Chemical Communications

IS - 7

ER -

A fast click-slow release strategy towards the HPLC-free synthesis of RNA

Abstract

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