A gene fusion system using the aminoglycoside 3′-phosphotransferase gene of the kanamycin-resistance transposon Tn903: use in the yeasts Kluyveromyces lactis and Saccharomyces cerevisiae

Chen Xin Jie; Fukuhara Hiroshi

doi:10.1016/0378-1119(88)90429-5

A gene fusion system using the aminoglycoside 3′-phosphotransferase gene of the kanamycin-resistance transposon Tn903: use in the yeasts Kluyveromyces lactis and Saccharomyces cerevisiae

Chen Xin Jie
, Fukuhara Hiroshi

Biochemistry & Molecular Biology

Upstate Medical University

Institut Curie

Research output: Contribution to journal › Article › peer-review

45 Scopus citations

Abstract

The aminoglycoside 3′-phosphotransferase type I (APHI)-coding gene of the bacterial transposon Tn903 confers resistance to kanamycin on bacteria and resistance to geneticin (G418) on many eukaryotes. We developed an APHI fusion system that can be used in the study of gene expression in these organisms, particularly in yeasts. The first 19 codons of the Km^R (APHI) gene can be deleted, and replaced by other genes in a continuous reading frame, without loss of APH activity. Examples of vector constructions are given which are adapted to the yeast Kluyveromyces lactis transformation system. Their derivatives containing the 2μ origin of replication can also be used in Saccharomyces cerevisiae.

Original language	English
Pages (from-to)	181-192
Number of pages	12
Journal	Gene
Volume	69
Issue number	2
DOIs	https://doi.org/10.1016/0378-1119(88)90429-5
State	Published - Sep 30 1988

Keywords

G418 (geneticin) resistance
Recombinant DNA
promoter probe
yeast shuttle vectors

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10.1016/0378-1119(88)90429-5

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title = "A gene fusion system using the aminoglycoside 3′-phosphotransferase gene of the kanamycin-resistance transposon Tn903: use in the yeasts Kluyveromyces lactis and Saccharomyces cerevisiae",

abstract = "The aminoglycoside 3′-phosphotransferase type I (APHI)-coding gene of the bacterial transposon Tn903 confers resistance to kanamycin on bacteria and resistance to geneticin (G418) on many eukaryotes. We developed an APHI fusion system that can be used in the study of gene expression in these organisms, particularly in yeasts. The first 19 codons of the KmR (APHI) gene can be deleted, and replaced by other genes in a continuous reading frame, without loss of APH activity. Examples of vector constructions are given which are adapted to the yeast Kluyveromyces lactis transformation system. Their derivatives containing the 2μ origin of replication can also be used in Saccharomyces cerevisiae.",

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AU - Hiroshi, Fukuhara

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N2 - The aminoglycoside 3′-phosphotransferase type I (APHI)-coding gene of the bacterial transposon Tn903 confers resistance to kanamycin on bacteria and resistance to geneticin (G418) on many eukaryotes. We developed an APHI fusion system that can be used in the study of gene expression in these organisms, particularly in yeasts. The first 19 codons of the KmR (APHI) gene can be deleted, and replaced by other genes in a continuous reading frame, without loss of APH activity. Examples of vector constructions are given which are adapted to the yeast Kluyveromyces lactis transformation system. Their derivatives containing the 2μ origin of replication can also be used in Saccharomyces cerevisiae.

AB - The aminoglycoside 3′-phosphotransferase type I (APHI)-coding gene of the bacterial transposon Tn903 confers resistance to kanamycin on bacteria and resistance to geneticin (G418) on many eukaryotes. We developed an APHI fusion system that can be used in the study of gene expression in these organisms, particularly in yeasts. The first 19 codons of the KmR (APHI) gene can be deleted, and replaced by other genes in a continuous reading frame, without loss of APH activity. Examples of vector constructions are given which are adapted to the yeast Kluyveromyces lactis transformation system. Their derivatives containing the 2μ origin of replication can also be used in Saccharomyces cerevisiae.

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A gene fusion system using the aminoglycoside 3′-phosphotransferase gene of the kanamycin-resistance transposon Tn903: use in the yeasts Kluyveromyces lactis and Saccharomyces cerevisiae

Abstract

Keywords

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