A gene‐cloning system for Kluyveromyces lactis and isolation of a chromosomal gene required for killer toxin production

X. J. Chen; M. Wésolowski‐Louvel; C. Tanguy‐Rougeau; M. M. Bianchi; L. Fabiani; M. Saliola; C. Falcone; L. Frontali; H. Fukuhara

doi:10.1002/jobm.3620280402

A gene‐cloning system for Kluyveromyces lactis and isolation of a chromosomal gene required for killer toxin production

X. J. Chen
, M. Wésolowski‐Louvel
, C. Tanguy‐Rougeau
, M. M. Bianchi
, L. Fabiani
, M. Saliola
, C. Falcone
, L. Frontali
, H. Fukuhara

Biochemistry & Molecular Biology

Upstate Medical University

Institut Curie
University of Rome La Sapienza

Research output: Contribution to journal › Review article › peer-review

20 Scopus citations

Abstract

A transformation system derived from the circular plasmid pKD1 has been developed for Kluyveromyces lactis. The principle is essentially equivalent to that of the 2 μ/Saccharomyces cerevisiae transformation system. The main features of the system are presented. Using a pKD1‐based DNA bank of K. lactis, the KEX1 gene involved in the killer system was isolated by complementation.

Original language	English
Pages (from-to)	211-220
Number of pages	10
Journal	Journal of Basic Microbiology
Volume	28
Issue number	4
DOIs	https://doi.org/10.1002/jobm.3620280402
State	Published - 1988

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title = "A gene‐cloning system for Kluyveromyces lactis and isolation of a chromosomal gene required for killer toxin production",

abstract = "A transformation system derived from the circular plasmid pKD1 has been developed for Kluyveromyces lactis. The principle is essentially equivalent to that of the 2 μ/Saccharomyces cerevisiae transformation system. The main features of the system are presented. Using a pKD1‐based DNA bank of K. lactis, the KEX1 gene involved in the killer system was isolated by complementation.",

author = "Chen, \{X. J.\} and M. W{\'e}solowski‐Louvel and C. Tanguy‐Rougeau and Bianchi, \{M. M.\} and L. Fabiani and M. Saliola and C. Falcone and L. Frontali and H. Fukuhara",

year = "1988",

doi = "10.1002/jobm.3620280402",

language = "English",

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pages = "211--220",

journal = "Journal of Basic Microbiology",

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T1 - A gene‐cloning system for Kluyveromyces lactis and isolation of a chromosomal gene required for killer toxin production

AU - Chen, X. J.

AU - Wésolowski‐Louvel, M.

AU - Tanguy‐Rougeau, C.

AU - Bianchi, M. M.

AU - Fabiani, L.

AU - Saliola, M.

AU - Falcone, C.

AU - Frontali, L.

AU - Fukuhara, H.

PY - 1988

Y1 - 1988

N2 - A transformation system derived from the circular plasmid pKD1 has been developed for Kluyveromyces lactis. The principle is essentially equivalent to that of the 2 μ/Saccharomyces cerevisiae transformation system. The main features of the system are presented. Using a pKD1‐based DNA bank of K. lactis, the KEX1 gene involved in the killer system was isolated by complementation.

AB - A transformation system derived from the circular plasmid pKD1 has been developed for Kluyveromyces lactis. The principle is essentially equivalent to that of the 2 μ/Saccharomyces cerevisiae transformation system. The main features of the system are presented. Using a pKD1‐based DNA bank of K. lactis, the KEX1 gene involved in the killer system was isolated by complementation.

UR - https://www.scopus.com/pages/publications/0024197924

U2 - 10.1002/jobm.3620280402

DO - 10.1002/jobm.3620280402

M3 - Review article

C2 - 3193361

SN - 0233-111X

VL - 28

SP - 211

EP - 220

JO - Journal of Basic Microbiology

JF - Journal of Basic Microbiology

IS - 4

ER -

A gene‐cloning system for Kluyveromyces lactis and isolation of a chromosomal gene required for killer toxin production

Abstract

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