TY - GEN
T1 - A handheld fluorometer for measuring cellular metabolism
AU - Nelson, Nicole
AU - Sander, David
AU - Dandin, Marc
AU - Sarje, Anshu
AU - Prakash, Somashekar
AU - Ji, Honghao
AU - Abshire, Pamela
PY - 2008
Y1 - 2008
N2 - We demonstrate the application of a handheld fluorometer optimized for UV excitable assays. We demonstrate the measurement of metabolic products as yeast cells germinate in dextrose solution. In particular we measure NADH which is produced during cellular respiration. The handheld fluorometer consists of a CMOS active pixel sensor with in-pixel CDS, coupled with a custom chromophore-polymer emission filter and a UV LED as the excitation source. The handheld fluorometer is able to detect as little as 10 μM of NADH, and in its present format should be applicable to any fluorescence assay with UV excitation and visible emission wavelengths.
AB - We demonstrate the application of a handheld fluorometer optimized for UV excitable assays. We demonstrate the measurement of metabolic products as yeast cells germinate in dextrose solution. In particular we measure NADH which is produced during cellular respiration. The handheld fluorometer consists of a CMOS active pixel sensor with in-pixel CDS, coupled with a custom chromophore-polymer emission filter and a UV LED as the excitation source. The handheld fluorometer is able to detect as little as 10 μM of NADH, and in its present format should be applicable to any fluorescence assay with UV excitation and visible emission wavelengths.
UR - https://www.scopus.com/pages/publications/51749093964
U2 - 10.1109/ISCAS.2008.4541609
DO - 10.1109/ISCAS.2008.4541609
M3 - Conference contribution
SN - 9781424416844
T3 - Proceedings - IEEE International Symposium on Circuits and Systems
SP - 1080
EP - 1083
BT - 2008 IEEE International Symposium on Circuits and Systems, ISCAS 2008
T2 - 2008 IEEE International Symposium on Circuits and Systems, ISCAS 2008
Y2 - 18 May 2008 through 21 May 2008
ER -