A near-infrared fluorescent aptananosensor enables selective detection of the stress hormone cortisol in artificial cerebrospinal fluid

Cortisol is a hormone which regulates the body's response to stressors. Detection and monitoring of cortisol levels can provide information about physical and psychological health, thus it is essential to develop a sensor that can detect it in a sensitive manner. This study presents a biocompatible near-infrared fluorescent sensor, wherein single-walled carbon nanotubes (SWCNT) are functionalized with a cortisol-specific aptamer. We found this sensor was capable of detecting cortisol from 37.5 μg mL⁻¹to 300 μg mL⁻¹and that it was selective for cortisol compared to the similar molecule estrogen. Moreover, SWCNT functionalized with non-specific oligonucleotides did not exhibit a concentration-dependent response to cortisol, demonstrating the specificity provided by the aptamer sequence. The sensor also demonstrated the ability to detect cortisol in artificial cerebrospinal fluid. We anticipate that future optimization of this sensor will enable potential point-of-care or implantable device-based rapid detection of cortisol, with the potential for improving overall patient health and stress.