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A novel cytometric tool for studying kinetics of nanoparticle uptake into cells

  • Virginia Polytechnic Institute and State University

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Therapeutic drug conjugated with nanoparticle for targeting delivery has rapidly gained interest in the area of nanomedicine. The nanocarriers, such as quantum dots (QD), are usually internalized into tumor cells through endocytic pathway. However, the study of nanoparticle endocytosis presents much challenge to conventional tools. Herein we use total internal reflection fluorescence flow cytometry (TIRF-FC) to investigate the process of QD uptake by cells at the single cell resolution. The kinetics of that process is also studied that the uptake speed through cell plasma membrane is a constant of 5.5 nm/min.

Original languageEnglish
Title of host publication14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Pages256-258
Number of pages3
StatePublished - 2010
Event14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands
Duration: Oct 3 2010Oct 7 2010

Publication series

Name14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Volume1

Conference

Conference14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Country/TerritoryNetherlands
CityGroningen
Period10/3/1010/7/10

Keywords

  • Microfluidic
  • Nanoparticle
  • Quantum dots
  • Total internal reflection fluorescence flow cytometry

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