@inproceedings{cf15d16388814f08b95e5f440a4fbd5f,
title = "A novel microfabricated device measures a large fraction of hormone release from individual-cells with high time resolution",
abstract = "Single-cell assays of hormone or neurotransmitter secretion have applications in cell-based biosensor development, high-throughput screening of drugs and basic science investigations. We are using microsystems technology to make electrochemical electrodes in picoliter-size wells for measuring catecholamine release from. individual cells. By making the well-electrode only slightly larger than the cell, high sensitivity and time resolution are achieved. The electrode can detect {"}spikes{"} of oxidative current corresponding to the release of individual catecholamine-containing vesicles from bovine adrenal chromaffin cells with millisecond time resolution. In, addition, we have simultaneously used whole-cell patch clamp recording to estimate that more than 60\% of catecholamine released from the cell is captured by the well-electrode.",
keywords = "amperometry, bioMEMS, electrochemistry, Exocytosis, neuroscience, patch-clamp, secretion, single-cell assays",
author = "Peng Chen and Bai Xu and N. Tokranova and Xiaojun Feng and J. Castracane and Gillis, \{K. D.\}",
note = "Publisher Copyright: {\textcopyright} 2002 IEEE.; 2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology ; Conference date: 02-05-2002 Through 04-05-2002",
year = "2002",
doi = "10.1109/MMB.2002.1002380",
language = "English",
series = "2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings",
publisher = "Institute of Electrical and Electronics Engineers Inc.",
pages = "481--484",
editor = "David Beebe and Andre Dittmar",
booktitle = "2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings",
}