Abstract
Injuring light induced structural changes in rod outer segment (ROS) membranes are studied using "ST EST spectroscopy" for spin labelled rhodopsin, ESR of lipid spin label and SDS gel-electrophoresis. Free SH-group content of rhodopsin and lipid peroxidation level were simultaneously determined as well. A decrease of rotational mobility of rhodopsin in ROS induced by prolonged illumination is shown to result from irreversible protein aggregation caused by disulfide bond formation between "hydrophobic" SH-groups of rhodopsin. Some decrease of lipid microviscosity and degree of order are found, in contrast to considerable rise in microviscosity due to Fe2+-ascorbate induced lipid peroxidation of ROS membranes. Lipid oxidation is found to accelerate protein aggregation which in its turn influences the state of lipid bilayer.
| Translated title of the contribution | Aggregation of rhodopsin molecules during damaging exposure of photoreceptor membranes to light |
|---|---|
| Original language | Russian |
| Pages (from-to) | 692-700 |
| Number of pages | 9 |
| Journal | Biofizika |
| Volume | 26 |
| Issue number | 4 |
| State | Published - Jul 1981 |
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