Analysis of a 1.6-μm circular plasmid from the yeast Kluyveromyces drosophilarum: Structure and molecular dimorphism

C. Falcone; M. Saliola; X. J. Chen; L. Frontali; H. Fukuhara

doi:10.1016/0147-619X(86)90044-2

Analysis of a 1.6-μm circular plasmid from the yeast Kluyveromyces drosophilarum: Structure and molecular dimorphism

C. Falcone
, M. Saliola
, X. J. Chen
, L. Frontali
, H. Fukuhara

Biochemistry & Molecular Biology

Upstate Medical University

University of Rome La Sapienza
Institut Curie

Research output: Contribution to journal › Article › peer-review

52 Scopus citations

Abstract

A new plasmid has been found in the yeast Kluyveromyces drosophilarum. It is a double-stranded circular DNA, 1.6 μm in length (4.8 kilobase pairs). As in the case of Saccharomyces 2μ circles, this plasmid occurs in two isomeric forms corresponding to the inversion of a segment between two 346-bp-long inverted repeats within the molecule. Each form has been separately cloned into bacterial plasmids. The new yeast plasmid, called pKD1, contains sequences that allow its replication in Saccharomyces cerevisiae.

Original language	English
Pages (from-to)	248-252
Number of pages	5
Journal	Plasmid
Volume	15
Issue number	3
DOIs	https://doi.org/10.1016/0147-619X(86)90044-2
State	Published - May 1986

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title = "Analysis of a 1.6-μm circular plasmid from the yeast Kluyveromyces drosophilarum: Structure and molecular dimorphism",

abstract = "A new plasmid has been found in the yeast Kluyveromyces drosophilarum. It is a double-stranded circular DNA, 1.6 μm in length (4.8 kilobase pairs). As in the case of Saccharomyces 2μ circles, this plasmid occurs in two isomeric forms corresponding to the inversion of a segment between two 346-bp-long inverted repeats within the molecule. Each form has been separately cloned into bacterial plasmids. The new yeast plasmid, called pKD1, contains sequences that allow its replication in Saccharomyces cerevisiae.",

author = "C. Falcone and M. Saliola and Chen, \{X. J.\} and L. Frontali and H. Fukuhara",

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AU - Saliola, M.

AU - Chen, X. J.

AU - Frontali, L.

AU - Fukuhara, H.

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AB - A new plasmid has been found in the yeast Kluyveromyces drosophilarum. It is a double-stranded circular DNA, 1.6 μm in length (4.8 kilobase pairs). As in the case of Saccharomyces 2μ circles, this plasmid occurs in two isomeric forms corresponding to the inversion of a segment between two 346-bp-long inverted repeats within the molecule. Each form has been separately cloned into bacterial plasmids. The new yeast plasmid, called pKD1, contains sequences that allow its replication in Saccharomyces cerevisiae.

UR - https://www.scopus.com/pages/publications/0022721323

U2 - 10.1016/0147-619X(86)90044-2

DO - 10.1016/0147-619X(86)90044-2

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Analysis of a 1.6-μm circular plasmid from the yeast Kluyveromyces drosophilarum: Structure and molecular dimorphism

Abstract

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