Analysis of DNA sequencing systems based on capillary electrophoresis

A. Stepukhovich; A. Tsupryk; O. Kosobokova; D. N. Gavrilov; B. Gorbovitski; G. Gudkov; G. Tyshko; M. Tcherevishnik; V. Gorfinkel

doi:10.1134/S1063784208060169

Analysis of DNA sequencing systems based on capillary electrophoresis

A. Stepukhovich
, A. Tsupryk
, O. Kosobokova
, D. N. Gavrilov
, B. Gorbovitski
, G. Gudkov
, G. Tyshko
, M. Tcherevishnik
, V. Gorfinkel

Electrical Engineering

Stony Brook University

Stony Brook University

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

A method for characterization and optimization of DNA sequencing systems based on capillary electrophoresis is proposed. A model is developed that relates the read length of a sequencing run and the parameters of DNA sequencing systems such as the properties of the separation medium, capillary length, injection time, concentration of the injected DNA sample, detection sensitivity, system noise, and the illumination power. The optimization of DNA sequencing systems will allow determination of optimum regimes for preparation, injection, and separation of DNA samples needed for achieving a read length required. The method proposed is applied to the characterization of capillary-electrophoretic DNA sequencing systems based on single-photon detection.

Original language	English
Pages (from-to)	763-775
Number of pages	13
Journal	Technical Physics
Volume	53
Issue number	6
DOIs	https://doi.org/10.1134/S1063784208060169
State	Published - Jun 2008

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title = "Analysis of DNA sequencing systems based on capillary electrophoresis",

abstract = "A method for characterization and optimization of DNA sequencing systems based on capillary electrophoresis is proposed. A model is developed that relates the read length of a sequencing run and the parameters of DNA sequencing systems such as the properties of the separation medium, capillary length, injection time, concentration of the injected DNA sample, detection sensitivity, system noise, and the illumination power. The optimization of DNA sequencing systems will allow determination of optimum regimes for preparation, injection, and separation of DNA samples needed for achieving a read length required. The method proposed is applied to the characterization of capillary-electrophoretic DNA sequencing systems based on single-photon detection.",

author = "A. Stepukhovich and A. Tsupryk and O. Kosobokova and Gavrilov, \{D. N.\} and B. Gorbovitski and G. Gudkov and G. Tyshko and M. Tcherevishnik and V. Gorfinkel",

year = "2008",

month = jun,

doi = "10.1134/S1063784208060169",

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pages = "763--775",

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T1 - Analysis of DNA sequencing systems based on capillary electrophoresis

AU - Stepukhovich, A.

AU - Tsupryk, A.

AU - Kosobokova, O.

AU - Gavrilov, D. N.

AU - Gorbovitski, B.

AU - Gudkov, G.

AU - Tyshko, G.

AU - Tcherevishnik, M.

AU - Gorfinkel, V.

PY - 2008/6

Y1 - 2008/6

N2 - A method for characterization and optimization of DNA sequencing systems based on capillary electrophoresis is proposed. A model is developed that relates the read length of a sequencing run and the parameters of DNA sequencing systems such as the properties of the separation medium, capillary length, injection time, concentration of the injected DNA sample, detection sensitivity, system noise, and the illumination power. The optimization of DNA sequencing systems will allow determination of optimum regimes for preparation, injection, and separation of DNA samples needed for achieving a read length required. The method proposed is applied to the characterization of capillary-electrophoretic DNA sequencing systems based on single-photon detection.

AB - A method for characterization and optimization of DNA sequencing systems based on capillary electrophoresis is proposed. A model is developed that relates the read length of a sequencing run and the parameters of DNA sequencing systems such as the properties of the separation medium, capillary length, injection time, concentration of the injected DNA sample, detection sensitivity, system noise, and the illumination power. The optimization of DNA sequencing systems will allow determination of optimum regimes for preparation, injection, and separation of DNA samples needed for achieving a read length required. The method proposed is applied to the characterization of capillary-electrophoretic DNA sequencing systems based on single-photon detection.

UR - https://www.scopus.com/pages/publications/46449107051

U2 - 10.1134/S1063784208060169

DO - 10.1134/S1063784208060169

M3 - Article

SN - 1063-7842

VL - 53

SP - 763

EP - 775

JO - Technical Physics

JF - Technical Physics

IS - 6

ER -

Analysis of DNA sequencing systems based on capillary electrophoresis

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