Abstract
The use of microcantilevers as a platform for the detection of aptamer-ligand interactions is investigated. A lead aptamer deoxyribozyme (aptazyme) was attached to the gold side of a microcantilever via thiol linkage. The aptazyme consists of a double-stranded DNA sequence containing one ribonucleotide; the dsDNA/RNA chimera is cleaved upon exposure to lead ions and the degree of cleavage is dependent upon the concentration of ligand. Specific detection of lead ions at the ten nanomolar level is demonstrated.
| Original language | English |
|---|---|
| Pages | 256-259 |
| Number of pages | 4 |
| State | Published - 2004 |
| Event | Chemical Sensors VI: Chemical and Biological Sensors and Analytical Methods - Proceedings of the International Symposium - Honolulu, HI, United States Duration: Oct 3 2004 → Oct 8 2004 |
Conference
| Conference | Chemical Sensors VI: Chemical and Biological Sensors and Analytical Methods - Proceedings of the International Symposium |
|---|---|
| Country/Territory | United States |
| City | Honolulu, HI |
| Period | 10/3/04 → 10/8/04 |
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