Assembly of microarrays for genome-wide measurement of DNA copy number

Antoine M. Snijders; Norma Nowak; Richard Segraves; Stephanie Blackwood; Nils Brown; Jeffrey Conroy; Greg Hamilton; Anna Katherine Hindle; Bing Huey; Karen Kimura; Sindy Law; Ken Myambo; Joel Palmer; Bauke Ylstra; Jingzhu Pearl Yue; Joe W. Gray; Ajay N. Jain; Daniel Pinkel; Donna G. Albertson

doi:10.1038/ng754

Assembly of microarrays for genome-wide measurement of DNA copy number

Antoine M. Snijders
, Norma Nowak
, Richard Segraves
, Stephanie Blackwood
, Nils Brown
, Jeffrey Conroy
, Greg Hamilton
, Anna Katherine Hindle
, Bing Huey
, Karen Kimura
, Sindy Law
, Ken Myambo
, Joel Palmer
, Bauke Ylstra
, Jingzhu Pearl Yue
, Joe W. Gray
, Ajay N. Jain
, Daniel Pinkel
, Donna G. Albertson

Biochemistry

University at Buffalo

University of California at San Francisco
Roswell Park Cancer Institute

Research output: Contribution to journal › Article › peer-review

796 Scopus citations

Abstract

We have assembled arrays of approximately 2,400 BAC clones for measurement of DNA copy number across the human genome. The arrays provide precise measurement (s.d. of log₂ ratios=0.05-0.10) in cell lines and clinical material, so that we can reliably detect and quantify high-level amplifications and single-copy alterations in diploid, polyploid and heterogeneous backgrounds.

Original language	English
Pages (from-to)	263-264
Number of pages	2
Journal	Nature Genetics
Volume	29
Issue number	3
DOIs	https://doi.org/10.1038/ng754
State	Published - 2001

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Snijders, A. M., Nowak, N., Segraves, R., Blackwood, S., Brown, N., Conroy, J., Hamilton, G., Hindle, A. K., Huey, B., Kimura, K., Law, S., Myambo, K., Palmer, J., Ylstra, B., Yue, J. P., Gray, J. W., Jain, A. N., Pinkel, D., & Albertson, D. G. (2001). Assembly of microarrays for genome-wide measurement of DNA copy number. Nature Genetics, 29(3), 263-264. https://doi.org/10.1038/ng754

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title = "Assembly of microarrays for genome-wide measurement of DNA copy number",

abstract = "We have assembled arrays of approximately 2,400 BAC clones for measurement of DNA copy number across the human genome. The arrays provide precise measurement (s.d. of log2 ratios=0.05-0.10) in cell lines and clinical material, so that we can reliably detect and quantify high-level amplifications and single-copy alterations in diploid, polyploid and heterogeneous backgrounds.",

author = "Snijders, \{Antoine M.\} and Norma Nowak and Richard Segraves and Stephanie Blackwood and Nils Brown and Jeffrey Conroy and Greg Hamilton and Hindle, \{Anna Katherine\} and Bing Huey and Karen Kimura and Sindy Law and Ken Myambo and Joel Palmer and Bauke Ylstra and Yue, \{Jingzhu Pearl\} and Gray, \{Joe W.\} and Jain, \{Ajay N.\} and Daniel Pinkel and Albertson, \{Donna G.\}",

year = "2001",

doi = "10.1038/ng754",

language = "English",

volume = "29",

pages = "263--264",

journal = "Nature Genetics",

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T1 - Assembly of microarrays for genome-wide measurement of DNA copy number

AU - Snijders, Antoine M.

AU - Nowak, Norma

AU - Segraves, Richard

AU - Blackwood, Stephanie

AU - Brown, Nils

AU - Conroy, Jeffrey

AU - Hamilton, Greg

AU - Hindle, Anna Katherine

AU - Huey, Bing

AU - Kimura, Karen

AU - Law, Sindy

AU - Myambo, Ken

AU - Palmer, Joel

AU - Ylstra, Bauke

AU - Yue, Jingzhu Pearl

AU - Gray, Joe W.

AU - Jain, Ajay N.

AU - Pinkel, Daniel

AU - Albertson, Donna G.

PY - 2001

Y1 - 2001

N2 - We have assembled arrays of approximately 2,400 BAC clones for measurement of DNA copy number across the human genome. The arrays provide precise measurement (s.d. of log2 ratios=0.05-0.10) in cell lines and clinical material, so that we can reliably detect and quantify high-level amplifications and single-copy alterations in diploid, polyploid and heterogeneous backgrounds.

AB - We have assembled arrays of approximately 2,400 BAC clones for measurement of DNA copy number across the human genome. The arrays provide precise measurement (s.d. of log2 ratios=0.05-0.10) in cell lines and clinical material, so that we can reliably detect and quantify high-level amplifications and single-copy alterations in diploid, polyploid and heterogeneous backgrounds.

UR - https://www.scopus.com/pages/publications/0035179871

U2 - 10.1038/ng754

DO - 10.1038/ng754

M3 - Article

C2 - 11687795

SN - 1061-4036

VL - 29

SP - 263

EP - 264

JO - Nature Genetics

JF - Nature Genetics

IS - 3

ER -

Assembly of microarrays for genome-wide measurement of DNA copy number

Abstract

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