Benzo(B)Thiophene-2-Carboxylic acid: Calcium uptake and cyclic AMP production in isolated bone cells

J. C. Robin; M. J. Brown; N. Weinfeld; R. M. Dziak

doi:10.1007/BF02405317

Benzo(B)Thiophene-2-Carboxylic acid: Calcium uptake and cyclic AMP production in isolated bone cells

J. C. Robin
, M. J. Brown
, N. Weinfeld
, R. M. Dziak

Department of Oral Biology

University at Buffalo

SUNY Buffalo

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

The purpose of the present study was to investigate the mechanism of action on bone of Benzo(B)Thiophene-2-Carboxylic Acid (BL-5583). BL-5583, at a dose range of 0.01-100 μg/ml, inhibited spontaneous as well as A23187 and PTH-induced bone resorption in tissue culture. This compound also decreased calcium uptake in both osteoclastic and osteoblastic enriched bone cell populations obtained by sequential collagenase digestion of 1-2 day newborn rat calvariae. The decrease occurred after a 5 min. incubation with⁴⁵Ca and BL-5583. The effective dose range was 0.01-100 μg/ml. No effect on leucine incorporation or lactic acid production by bone cells was observed. BL-5583 also induced a transient decrease in calcium uptake in skin cells isolated from fetal rats by collagenase digestion, suggesting a lack of tissue specificity for this compound. No effect on cyclic AMP in isolated bone cells was observed with the same dose range that produced a calcium effect.

Original language	English
Pages (from-to)	194-199
Number of pages	6
Journal	Calcified Tissue International
Volume	36
Issue number	1
DOIs	https://doi.org/10.1007/BF02405317
State	Published - Dec 1984

Keywords

Benzo(B)Thiophene-2-Carboxylic Acid
Bone cells
Calcium
Cyclic AMP

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10.1007/BF02405317

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title = "Benzo(B)Thiophene-2-Carboxylic acid: Calcium uptake and cyclic AMP production in isolated bone cells",

abstract = "The purpose of the present study was to investigate the mechanism of action on bone of Benzo(B)Thiophene-2-Carboxylic Acid (BL-5583). BL-5583, at a dose range of 0.01-100 μg/ml, inhibited spontaneous as well as A23187 and PTH-induced bone resorption in tissue culture. This compound also decreased calcium uptake in both osteoclastic and osteoblastic enriched bone cell populations obtained by sequential collagenase digestion of 1-2 day newborn rat calvariae. The decrease occurred after a 5 min. incubation with45Ca and BL-5583. The effective dose range was 0.01-100 μg/ml. No effect on leucine incorporation or lactic acid production by bone cells was observed. BL-5583 also induced a transient decrease in calcium uptake in skin cells isolated from fetal rats by collagenase digestion, suggesting a lack of tissue specificity for this compound. No effect on cyclic AMP in isolated bone cells was observed with the same dose range that produced a calcium effect.",

keywords = "Benzo(B)Thiophene-2-Carboxylic Acid, Bone cells, Calcium, Cyclic AMP",

author = "Robin, \{J. C.\} and Brown, \{M. J.\} and N. Weinfeld and Dziak, \{R. M.\}",

year = "1984",

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doi = "10.1007/BF02405317",

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journal = "Calcified Tissue International",

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T2 - Calcium uptake and cyclic AMP production in isolated bone cells

AU - Robin, J. C.

AU - Brown, M. J.

AU - Weinfeld, N.

AU - Dziak, R. M.

PY - 1984/12

Y1 - 1984/12

N2 - The purpose of the present study was to investigate the mechanism of action on bone of Benzo(B)Thiophene-2-Carboxylic Acid (BL-5583). BL-5583, at a dose range of 0.01-100 μg/ml, inhibited spontaneous as well as A23187 and PTH-induced bone resorption in tissue culture. This compound also decreased calcium uptake in both osteoclastic and osteoblastic enriched bone cell populations obtained by sequential collagenase digestion of 1-2 day newborn rat calvariae. The decrease occurred after a 5 min. incubation with45Ca and BL-5583. The effective dose range was 0.01-100 μg/ml. No effect on leucine incorporation or lactic acid production by bone cells was observed. BL-5583 also induced a transient decrease in calcium uptake in skin cells isolated from fetal rats by collagenase digestion, suggesting a lack of tissue specificity for this compound. No effect on cyclic AMP in isolated bone cells was observed with the same dose range that produced a calcium effect.

AB - The purpose of the present study was to investigate the mechanism of action on bone of Benzo(B)Thiophene-2-Carboxylic Acid (BL-5583). BL-5583, at a dose range of 0.01-100 μg/ml, inhibited spontaneous as well as A23187 and PTH-induced bone resorption in tissue culture. This compound also decreased calcium uptake in both osteoclastic and osteoblastic enriched bone cell populations obtained by sequential collagenase digestion of 1-2 day newborn rat calvariae. The decrease occurred after a 5 min. incubation with45Ca and BL-5583. The effective dose range was 0.01-100 μg/ml. No effect on leucine incorporation or lactic acid production by bone cells was observed. BL-5583 also induced a transient decrease in calcium uptake in skin cells isolated from fetal rats by collagenase digestion, suggesting a lack of tissue specificity for this compound. No effect on cyclic AMP in isolated bone cells was observed with the same dose range that produced a calcium effect.

KW - Benzo(B)Thiophene-2-Carboxylic Acid

KW - Bone cells

KW - Calcium

KW - Cyclic AMP

UR - https://www.scopus.com/pages/publications/0021191416

U2 - 10.1007/BF02405317

DO - 10.1007/BF02405317

M3 - Article

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SN - 0171-967X

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JO - Calcified Tissue International

JF - Calcified Tissue International

IS - 1

ER -

Benzo(B)Thiophene-2-Carboxylic acid: Calcium uptake and cyclic AMP production in isolated bone cells

Abstract

Keywords

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