Bio-orthogonal chemistry enables solid phase synthesis and HPLC and gel-free purification of long RNA oligonucleotides

Muhan He; Xunshen Wu; Song Mao; Phensinee Haruehanroengra; Irfan Khan; Jia Sheng; Maksim Royzen

doi:10.1039/d1cc00096a

Bio-orthogonal chemistry enables solid phase synthesis and HPLC and gel-free purification of long RNA oligonucleotides

Muhan He
, Xunshen Wu
, Song Mao
, Phensinee Haruehanroengra
, Irfan Khan
, Jia Sheng
, Maksim Royzen

Chemistry

University at Albany

SUNY Albany

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Solid phase synthesis of RNA oligonucleotides which are over 100-nt in length remains challenging due to the complexity of purification of the target strand from failure sequences. This work describes a non-chromatographic strategy that will enable routine solid phase synthesis of long RNA strands.

Original language	English
Pages (from-to)	4263-4266
Number of pages	4
Journal	Chemical Communications
Volume	57
Issue number	35
DOIs	https://doi.org/10.1039/d1cc00096a
State	Published - May 2 2021

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title = "Bio-orthogonal chemistry enables solid phase synthesis and HPLC and gel-free purification of long RNA oligonucleotides",

abstract = "Solid phase synthesis of RNA oligonucleotides which are over 100-nt in length remains challenging due to the complexity of purification of the target strand from failure sequences. This work describes a non-chromatographic strategy that will enable routine solid phase synthesis of long RNA strands.",

author = "Muhan He and Xunshen Wu and Song Mao and Phensinee Haruehanroengra and Irfan Khan and Jia Sheng and Maksim Royzen",

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T1 - Bio-orthogonal chemistry enables solid phase synthesis and HPLC and gel-free purification of long RNA oligonucleotides

AU - He, Muhan

AU - Wu, Xunshen

AU - Mao, Song

AU - Haruehanroengra, Phensinee

AU - Khan, Irfan

AU - Sheng, Jia

AU - Royzen, Maksim

PY - 2021/5/2

Y1 - 2021/5/2

N2 - Solid phase synthesis of RNA oligonucleotides which are over 100-nt in length remains challenging due to the complexity of purification of the target strand from failure sequences. This work describes a non-chromatographic strategy that will enable routine solid phase synthesis of long RNA strands.

AB - Solid phase synthesis of RNA oligonucleotides which are over 100-nt in length remains challenging due to the complexity of purification of the target strand from failure sequences. This work describes a non-chromatographic strategy that will enable routine solid phase synthesis of long RNA strands.

UR - https://www.scopus.com/pages/publications/85104946009

U2 - 10.1039/d1cc00096a

DO - 10.1039/d1cc00096a

M3 - Article

C2 - 33913974

SN - 1359-7345

VL - 57

SP - 4263

EP - 4266

JO - Chemical Communications

JF - Chemical Communications

IS - 35

ER -

Bio-orthogonal chemistry enables solid phase synthesis and HPLC and gel-free purification of long RNA oligonucleotides

Abstract

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