Calreticulin, destrin, argininosuccinate synthase and phosphoglycerate kinase are down-regulated in the cytosol compartment of the melphalan resistant MCF-7 cell line

Yetrib Hathout; Kelly Riordan; Marion Gehrmann; Catherine Fenselau

Calreticulin, destrin, argininosuccinate synthase and phosphoglycerate kinase are down-regulated in the cytosol compartment of the melphalan resistant MCF-7 cell line

Yetrib Hathout
, Kelly Riordan
, Marion Gehrmann
, Catherine Fenselau

Pharmaceutical Sciences

Binghamton University

University of Maryland, College Park

Research output: Contribution to conference › Paper › peer-review

Abstract

The differential protein expression was studied focusing on down regulation in the cytosol fraction of a breast cancer MCF-7 cell line selected for resistance toward the alkylating agent melphalan. A combination of cell fractionation, two dimensional gel electrophoresis, mass spectrometry and bioinformatics was used during the study. MCF-7 control and melphalan resistant MCF-7 cells were grown to confluence in serum supplemented media. Comparative image analysis of the protein patterns in the cytosol fraction of MCF-7 control and MelR MCF-7 revealed several proteins whose expression was altered between the two cell lines.

Original language	English
Pages	271-272
Number of pages	2
State	Published - 2002
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002

Conference

Conference	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	06/2/02 → 06/6/02

Cite this

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title = "Calreticulin, destrin, argininosuccinate synthase and phosphoglycerate kinase are down-regulated in the cytosol compartment of the melphalan resistant MCF-7 cell line",

abstract = "The differential protein expression was studied focusing on down regulation in the cytosol fraction of a breast cancer MCF-7 cell line selected for resistance toward the alkylating agent melphalan. A combination of cell fractionation, two dimensional gel electrophoresis, mass spectrometry and bioinformatics was used during the study. MCF-7 control and melphalan resistant MCF-7 cells were grown to confluence in serum supplemented media. Comparative image analysis of the protein patterns in the cytosol fraction of MCF-7 control and MelR MCF-7 revealed several proteins whose expression was altered between the two cell lines.",

author = "Yetrib Hathout and Kelly Riordan and Marion Gehrmann and Catherine Fenselau",

year = "2002",

language = "English",

pages = "271--272",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

}

Hathout, Y, Riordan, K, Gehrmann, M & Fenselau, C 2002, 'Calreticulin, destrin, argininosuccinate synthase and phosphoglycerate kinase are down-regulated in the cytosol compartment of the melphalan resistant MCF-7 cell line', Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 06/2/02 - 06/6/02 pp. 271-272.

Calreticulin, destrin, argininosuccinate synthase and phosphoglycerate kinase are down-regulated in the cytosol compartment of the melphalan resistant MCF-7 cell line. / Hathout, Yetrib; Riordan, Kelly; Gehrmann, Marion et al.
2002. 271-272 Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.

Research output: Contribution to conference › Paper › peer-review

TY - CONF

T1 - Calreticulin, destrin, argininosuccinate synthase and phosphoglycerate kinase are down-regulated in the cytosol compartment of the melphalan resistant MCF-7 cell line

AU - Hathout, Yetrib

AU - Riordan, Kelly

AU - Gehrmann, Marion

AU - Fenselau, Catherine

PY - 2002

Y1 - 2002

N2 - The differential protein expression was studied focusing on down regulation in the cytosol fraction of a breast cancer MCF-7 cell line selected for resistance toward the alkylating agent melphalan. A combination of cell fractionation, two dimensional gel electrophoresis, mass spectrometry and bioinformatics was used during the study. MCF-7 control and melphalan resistant MCF-7 cells were grown to confluence in serum supplemented media. Comparative image analysis of the protein patterns in the cytosol fraction of MCF-7 control and MelR MCF-7 revealed several proteins whose expression was altered between the two cell lines.

AB - The differential protein expression was studied focusing on down regulation in the cytosol fraction of a breast cancer MCF-7 cell line selected for resistance toward the alkylating agent melphalan. A combination of cell fractionation, two dimensional gel electrophoresis, mass spectrometry and bioinformatics was used during the study. MCF-7 control and melphalan resistant MCF-7 cells were grown to confluence in serum supplemented media. Comparative image analysis of the protein patterns in the cytosol fraction of MCF-7 control and MelR MCF-7 revealed several proteins whose expression was altered between the two cell lines.

UR - https://www.scopus.com/pages/publications/2442673075

M3 - Paper

SP - 271

EP - 272

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

Calreticulin, destrin, argininosuccinate synthase and phosphoglycerate kinase are down-regulated in the cytosol compartment of the melphalan resistant MCF-7 cell line

Abstract

Conference

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