@inproceedings{94aa24b568d646aa9046aae891177efc,
title = "Cell motion in shear flow combined with Dean vortices strongly affects DNA transfer during flow-through electroporation",
abstract = "Electroporation is widely used to generate nanoscale pores within the membrane under an external electric field, enabling uptake of genes. However, in a typical electroporation process, only a small fraction of the cell membrane is actually permeabilized to an extent that contributes to transfection. In here we harness inertial Dean flow effects that arise when the suspended cells are transported through a curved spiral-shaped microchannel to overcome this limitation. We observe a two-fold enhancement in transfection efficiency using this device, as compared with a straight microchannel. These results suggest a new and simplified approach to achieve greatly enhanced gene delivery by electroporation in a continuous high-throughput format.",
keywords = "Dean flow, Flow-through electroporation, Microfluidic, Transfection",
author = "Jun Wang and Yihong Zhan and Ugaz, \{Victor M.\} and Chang Lu",
year = "2009",
language = "English",
isbn = "9780979806421",
series = "Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
publisher = "Chemical and Biological Microsystems Society",
pages = "1180--1182",
booktitle = "Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
note = "13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 ; Conference date: 01-11-2009 Through 05-11-2009",
}