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Cell segmentation via spectral analysis on phase retardation features

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

4 Scopus citations

Abstract

In this paper, the authors propose a cell segmentation algorithm via spectral analysis over phase retardation features, which are derived from the optical principle of phase contrast microscopy image formation process. Images are first partitioned into phase-homogeneous atoms by clustering neighboring pixels with similar phase retardation features. Cell segmentation is then performed by clustering the atoms into several clusters using multi-class spectral analysis. Experimental results demonstrate that our method generates quality cell segmentation results and outperforms previous methods.

Original languageEnglish
Title of host publicationISBI 2013 - 2013 IEEE 10th International Symposium on Biomedical Imaging
Subtitle of host publicationFrom Nano to Macro
PublisherIEEE Computer Society
Pages1477-1483
Number of pages7
ISBN (Print)9781467364546
DOIs
StatePublished - 2013
Event10th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2013 - San Francisco, CA, United States
Duration: Apr 7 2013Apr 11 2013

Publication series

NameProceedings - International Symposium on Biomedical Imaging

Conference

Conference10th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2013
Country/TerritoryUnited States
CitySan Francisco, CA
Period04/7/1304/11/13

Keywords

  • cell segmentation
  • phase contrast microscopy image analysis
  • phase retardation feature
  • spectral analysis

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