Cgrlp, a novel nucleolar protein encoded by saccharomyces cerevisiae orf YGL0292W

Jianlan Sun; Molly McFarland; Douglas Boettner; John Panepinto; Judith C. Rhodes; David S. Askew

doi:10.1007/s002840010180

Cgrlp, a novel nucleolar protein encoded by saccharomyces cerevisiae orf YGL0292W

Jianlan Sun
, Molly McFarland
, Douglas Boettner
, John Panepinto
, Judith C. Rhodes
, David S. Askew

Microbiology and Immunology

University at Buffalo

University of Cincinnati

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Saccharomyces cerevisiae open reading frame (ORF) YGL029w (CGR1) encodes a small hydrophilic protein of unknown function. To investigate the role of this gene, we have determined the intracellular localization of the encoded product and examined the effects of Cgrlp depletion on cell growth. Tagging Cgrlp with the green fluorescent protein (GFP) or the myc epitope showed focal accumulation of the fusion protein in the yeast nucleolus, and this localization overlapped with the distribution of the nucleolar protein Noplp. Cells depleted of CGR1 mRNA were growth impaired and hypersensitive to the translational inhibitor paromomycin, and this phenotype was complemented by episomal expression of the CGR1-GFP fusion gene. These results identify Cgrlp as a novel component of the yeast nucleolus and suggest a potential role in ribosome biogenesis.

Original language	English
Pages (from-to)	65-69
Number of pages	5
Journal	Current Microbiology
Volume	42
Issue number	1
DOIs	https://doi.org/10.1007/s002840010180
State	Published - 2001

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title = "Cgrlp, a novel nucleolar protein encoded by saccharomyces cerevisiae orf YGL0292W",

abstract = "Saccharomyces cerevisiae open reading frame (ORF) YGL029w (CGR1) encodes a small hydrophilic protein of unknown function. To investigate the role of this gene, we have determined the intracellular localization of the encoded product and examined the effects of Cgrlp depletion on cell growth. Tagging Cgrlp with the green fluorescent protein (GFP) or the myc epitope showed focal accumulation of the fusion protein in the yeast nucleolus, and this localization overlapped with the distribution of the nucleolar protein Noplp. Cells depleted of CGR1 mRNA were growth impaired and hypersensitive to the translational inhibitor paromomycin, and this phenotype was complemented by episomal expression of the CGR1-GFP fusion gene. These results identify Cgrlp as a novel component of the yeast nucleolus and suggest a potential role in ribosome biogenesis.",

author = "Jianlan Sun and Molly McFarland and Douglas Boettner and John Panepinto and Rhodes, \{Judith C.\} and Askew, \{David S.\}",

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T1 - Cgrlp, a novel nucleolar protein encoded by saccharomyces cerevisiae orf YGL0292W

AU - Sun, Jianlan

AU - McFarland, Molly

AU - Boettner, Douglas

AU - Panepinto, John

AU - Rhodes, Judith C.

AU - Askew, David S.

PY - 2001

Y1 - 2001

N2 - Saccharomyces cerevisiae open reading frame (ORF) YGL029w (CGR1) encodes a small hydrophilic protein of unknown function. To investigate the role of this gene, we have determined the intracellular localization of the encoded product and examined the effects of Cgrlp depletion on cell growth. Tagging Cgrlp with the green fluorescent protein (GFP) or the myc epitope showed focal accumulation of the fusion protein in the yeast nucleolus, and this localization overlapped with the distribution of the nucleolar protein Noplp. Cells depleted of CGR1 mRNA were growth impaired and hypersensitive to the translational inhibitor paromomycin, and this phenotype was complemented by episomal expression of the CGR1-GFP fusion gene. These results identify Cgrlp as a novel component of the yeast nucleolus and suggest a potential role in ribosome biogenesis.

AB - Saccharomyces cerevisiae open reading frame (ORF) YGL029w (CGR1) encodes a small hydrophilic protein of unknown function. To investigate the role of this gene, we have determined the intracellular localization of the encoded product and examined the effects of Cgrlp depletion on cell growth. Tagging Cgrlp with the green fluorescent protein (GFP) or the myc epitope showed focal accumulation of the fusion protein in the yeast nucleolus, and this localization overlapped with the distribution of the nucleolar protein Noplp. Cells depleted of CGR1 mRNA were growth impaired and hypersensitive to the translational inhibitor paromomycin, and this phenotype was complemented by episomal expression of the CGR1-GFP fusion gene. These results identify Cgrlp as a novel component of the yeast nucleolus and suggest a potential role in ribosome biogenesis.

UR - https://www.scopus.com/pages/publications/0034745490

U2 - 10.1007/s002840010180

DO - 10.1007/s002840010180

M3 - Article

C2 - 11116400

SN - 0343-8651

VL - 42

SP - 65

EP - 69

JO - Current Microbiology

JF - Current Microbiology

IS - 1

ER -

Cgrlp, a novel nucleolar protein encoded by saccharomyces cerevisiae orf YGL0292W

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