Abstract
ATP-sensitive potassium channels (K(ATP)) are widely expressed and yet little is known about the mechanisms regulating their expression. Here we report that expression of chick heart Kir6.1 is regulated by channel activators. Activation of K(ATP) with either ATP depletion or pinacidil, up-regulated Kir6.1 mRNA. 1.8- to 2.4-fold in cultured ventricular myocytes as measured by competitive PCR. Pinacidil treatment also increased Kir6.1 protein as detected using an antibody to Kir6.1. Glibenclamide, a K(ATP) inhibitor, completely blocked the pinacidil-induced increase in Kir6.1 levels. It appears that Kir6.1 is up-regulated by an unknown signal transduction pathway initiated by K(ATP) opening.
| Original language | English |
|---|---|
| Pages (from-to) | 121-125 |
| Number of pages | 5 |
| Journal | FEBS Letters |
| Volume | 412 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jul 21 1997 |
Keywords
- ATP sensitive
- Cardiac ion channel
- Pinacidil
- Potassium channel
- Sulfonylurea
- mRNA regulation
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