Chromosomal Localization of M/SAR from DNA Clone λ20p1.4 in Drosophila melanogaster Salivary Glands

M/SAR of Drosophila melanogaster DNA clone λ20p1.4 was localized on chromosomes by in situ hybridization of the λ20p1.4 DNA fragment and its deletion mutants with salivary gland chromosomes of third instar larvae of the lines Canton S and Oregon R. The M/SAR sequence, capable of specifically binding to lamin in vitro, was detected in the pericentric regions 20CD, 40EF, 41AB, the proximal region of chromosome 3, and region 101 of chromosome 4, which attach to the nuclear envelope in cells of D. melanogaster salivary glands, and in region 49D, which does not come into contact with the nuclear periphery. This is the first M/SAR localized in pericentric chromatin of D. melanogaster.