Cleavage of DNA by nickel complexes

Janet R. Morrow; Kimberly A. Kolasa

doi:10.1016/S0020-1693(00)85319-0

Cleavage of DNA by nickel complexes

Janet R. Morrow
, Kimberly A. Kolasa

Chemistry

University at Buffalo

SUNY Buffalo

Research output: Contribution to journal › Article › peer-review

46 Scopus citations

Abstract

The cleavage of plasmid DNA (pUB110) by several square planar nickel(II) complexes in the presence of either magnesium monoperoxyphthalic acid (MPPA) or iodosylbenzene was investigated. At 25°C and near neutral pH, Ni(salen) (100 μM) or Ni(CR)²⁺ (100 μM) promoted complete conversion of supercoiled plasmid to the nicked circular form in 5 min with iodosylbenzene (0.1 g/ml) as oxidant or in 2.5 h with MPPA (1 mM) as oxidant (salen = bis(salicylaldehyde)ethylenediimine, CR = 2,12-dimethyl-3,7,11,17-tetraazabicyclo[11.3.1]heptadeca- 1(17),2,11,13,15-pentaene). No cleavage was observed under similar conditions with Ni(cyclam)²⁺, Ni(dioxocyclam), Ni(TPP) or Ni(NO₃)₂ (cyclam = 1,4,8,11-tetraazacyclotetradecane, dioxocyclam = 1,4,8,11-tetraazacyclotetradecane- 5,7-dione, TPP=5,10,15,20-tetraphenyl-21H,23H-porphine). Possible roles for the nickel complexes in promoting DNA cleavage are discussed.

Original language	English
Pages (from-to)	245-248
Number of pages	4
Journal	Inorganica Chimica Acta
Volume	195
Issue number	2
DOIs	https://doi.org/10.1016/S0020-1693(00)85319-0
State	Published - May 15 1992

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title = "Cleavage of DNA by nickel complexes",

abstract = "The cleavage of plasmid DNA (pUB110) by several square planar nickel(II) complexes in the presence of either magnesium monoperoxyphthalic acid (MPPA) or iodosylbenzene was investigated. At 25°C and near neutral pH, Ni(salen) (100 μM) or Ni(CR)2+ (100 μM) promoted complete conversion of supercoiled plasmid to the nicked circular form in 5 min with iodosylbenzene (0.1 g/ml) as oxidant or in 2.5 h with MPPA (1 mM) as oxidant (salen = bis(salicylaldehyde)ethylenediimine, CR = 2,12-dimethyl-3,7,11,17-tetraazabicyclo[11.3.1]heptadeca- 1(17),2,11,13,15-pentaene). No cleavage was observed under similar conditions with Ni(cyclam)2+, Ni(dioxocyclam), Ni(TPP) or Ni(NO3)2 (cyclam = 1,4,8,11-tetraazacyclotetradecane, dioxocyclam = 1,4,8,11-tetraazacyclotetradecane- 5,7-dione, TPP=5,10,15,20-tetraphenyl-21H,23H-porphine). Possible roles for the nickel complexes in promoting DNA cleavage are discussed.",

author = "Morrow, \{Janet R.\} and Kolasa, \{Kimberly A.\}",

year = "1992",

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doi = "10.1016/S0020-1693(00)85319-0",

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AU - Morrow, Janet R.

AU - Kolasa, Kimberly A.

PY - 1992/5/15

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N2 - The cleavage of plasmid DNA (pUB110) by several square planar nickel(II) complexes in the presence of either magnesium monoperoxyphthalic acid (MPPA) or iodosylbenzene was investigated. At 25°C and near neutral pH, Ni(salen) (100 μM) or Ni(CR)2+ (100 μM) promoted complete conversion of supercoiled plasmid to the nicked circular form in 5 min with iodosylbenzene (0.1 g/ml) as oxidant or in 2.5 h with MPPA (1 mM) as oxidant (salen = bis(salicylaldehyde)ethylenediimine, CR = 2,12-dimethyl-3,7,11,17-tetraazabicyclo[11.3.1]heptadeca- 1(17),2,11,13,15-pentaene). No cleavage was observed under similar conditions with Ni(cyclam)2+, Ni(dioxocyclam), Ni(TPP) or Ni(NO3)2 (cyclam = 1,4,8,11-tetraazacyclotetradecane, dioxocyclam = 1,4,8,11-tetraazacyclotetradecane- 5,7-dione, TPP=5,10,15,20-tetraphenyl-21H,23H-porphine). Possible roles for the nickel complexes in promoting DNA cleavage are discussed.

AB - The cleavage of plasmid DNA (pUB110) by several square planar nickel(II) complexes in the presence of either magnesium monoperoxyphthalic acid (MPPA) or iodosylbenzene was investigated. At 25°C and near neutral pH, Ni(salen) (100 μM) or Ni(CR)2+ (100 μM) promoted complete conversion of supercoiled plasmid to the nicked circular form in 5 min with iodosylbenzene (0.1 g/ml) as oxidant or in 2.5 h with MPPA (1 mM) as oxidant (salen = bis(salicylaldehyde)ethylenediimine, CR = 2,12-dimethyl-3,7,11,17-tetraazabicyclo[11.3.1]heptadeca- 1(17),2,11,13,15-pentaene). No cleavage was observed under similar conditions with Ni(cyclam)2+, Ni(dioxocyclam), Ni(TPP) or Ni(NO3)2 (cyclam = 1,4,8,11-tetraazacyclotetradecane, dioxocyclam = 1,4,8,11-tetraazacyclotetradecane- 5,7-dione, TPP=5,10,15,20-tetraphenyl-21H,23H-porphine). Possible roles for the nickel complexes in promoting DNA cleavage are discussed.

UR - https://www.scopus.com/pages/publications/0000932646

U2 - 10.1016/S0020-1693(00)85319-0

DO - 10.1016/S0020-1693(00)85319-0

M3 - Article

SN - 0020-1693

VL - 195

SP - 245

EP - 248

JO - Inorganica Chimica Acta

JF - Inorganica Chimica Acta

IS - 2

ER -

Cleavage of DNA by nickel complexes

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