Coding potential of laboratory and clinical strains of human cytomegalovirus

Eain Murphy; Dong Yu; Jane Grimwood; Jeremy Schmutz; Mark Dickson; Michael A. Jarvis; Gabriele Hahn; Jay A. Nelson; Richard M. Myers; Thomas E. Shenk

doi:10.1073/pnas.2136652100

Coding potential of laboratory and clinical strains of human cytomegalovirus

Eain Murphy
, Dong Yu
, Jane Grimwood
, Jeremy Schmutz
, Mark Dickson
, Michael A. Jarvis
, Gabriele Hahn
, Jay A. Nelson
, Richard M. Myers
, Thomas E. Shenk

Microbiology & Immunology

Upstate Medical University

Princeton University
Stanford University
Oregon Health and Science University
Ludwig Maximilian University of Munich

Research output: Contribution to journal › Article › peer-review

445 Scopus citations

Abstract

Six strains of human cytomegalovirus have been sequenced, including two laboratory strains (AD169 and Towne) that have been extensively passaged in fibroblasts and four clinical isolates that have been passaged to a limited extent in the laboratory (Toledo, FIX, PH, and TR). All of the sequenced viral genomes have been cloned as infectious bacterial artificial chromosomes. A total of 252 ORFs with the potential to encode proteins have been identified that are conserved in all four clinical isolates of the virus. Multiple sequence alignments revealed substantial variation in the amino acid sequences encoded by many of the conserved ORFs.

Original language	English
Pages (from-to)	14976-14981
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	100
Issue number	25
DOIs	https://doi.org/10.1073/pnas.2136652100
State	Published - Dec 9 2003

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title = "Coding potential of laboratory and clinical strains of human cytomegalovirus",

abstract = "Six strains of human cytomegalovirus have been sequenced, including two laboratory strains (AD169 and Towne) that have been extensively passaged in fibroblasts and four clinical isolates that have been passaged to a limited extent in the laboratory (Toledo, FIX, PH, and TR). All of the sequenced viral genomes have been cloned as infectious bacterial artificial chromosomes. A total of 252 ORFs with the potential to encode proteins have been identified that are conserved in all four clinical isolates of the virus. Multiple sequence alignments revealed substantial variation in the amino acid sequences encoded by many of the conserved ORFs.",

author = "Eain Murphy and Dong Yu and Jane Grimwood and Jeremy Schmutz and Mark Dickson and Jarvis, \{Michael A.\} and Gabriele Hahn and Nelson, \{Jay A.\} and Myers, \{Richard M.\} and Shenk, \{Thomas E.\}",

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doi = "10.1073/pnas.2136652100",

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T1 - Coding potential of laboratory and clinical strains of human cytomegalovirus

AU - Murphy, Eain

AU - Yu, Dong

AU - Grimwood, Jane

AU - Schmutz, Jeremy

AU - Dickson, Mark

AU - Jarvis, Michael A.

AU - Hahn, Gabriele

AU - Nelson, Jay A.

AU - Myers, Richard M.

AU - Shenk, Thomas E.

PY - 2003/12/9

Y1 - 2003/12/9

N2 - Six strains of human cytomegalovirus have been sequenced, including two laboratory strains (AD169 and Towne) that have been extensively passaged in fibroblasts and four clinical isolates that have been passaged to a limited extent in the laboratory (Toledo, FIX, PH, and TR). All of the sequenced viral genomes have been cloned as infectious bacterial artificial chromosomes. A total of 252 ORFs with the potential to encode proteins have been identified that are conserved in all four clinical isolates of the virus. Multiple sequence alignments revealed substantial variation in the amino acid sequences encoded by many of the conserved ORFs.

AB - Six strains of human cytomegalovirus have been sequenced, including two laboratory strains (AD169 and Towne) that have been extensively passaged in fibroblasts and four clinical isolates that have been passaged to a limited extent in the laboratory (Toledo, FIX, PH, and TR). All of the sequenced viral genomes have been cloned as infectious bacterial artificial chromosomes. A total of 252 ORFs with the potential to encode proteins have been identified that are conserved in all four clinical isolates of the virus. Multiple sequence alignments revealed substantial variation in the amino acid sequences encoded by many of the conserved ORFs.

UR - https://www.scopus.com/pages/publications/10744225986

U2 - 10.1073/pnas.2136652100

DO - 10.1073/pnas.2136652100

M3 - Article

C2 - 14657367

SN - 0027-8424

VL - 100

SP - 14976

EP - 14981

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 25

ER -

Coding potential of laboratory and clinical strains of human cytomegalovirus

Abstract

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