Comparison of maximum length sequence and randomized stimulation and averaging methods on the bottlenose dolphin auditory brainstem response

The purpose of the present study was to compare auditory brainstem responses (ABRs) using two approaches that allow the use of high stimulation rates, but with different temporal variability in the interstimulus interval: maximum length sequences (MLS) and iterative randomized stimulation and averaging (I-RSA). ABRs were obtained to click stimuli in six bottlenose dolphins (Tursiops truncatus). In experiment 1, click level was held constant and click rate varied from 25 to 1250 Hz. For MLS, interstimulus intervals varied by a factor of 6 at each rate, while for I-RSA the interstimulus intervals varied by ± 0.5 ms regardless of rate. In experiment 2, stimulus rates ranged from 100 to 1000 Hz and click level varied from 105 to 135 dB re: 1 μPa. For experiment 1, MLS and I-RSA showed similar decreases in ABR peak amplitudes and increases in ABR peak latencies and interwave intervals with increasing rate. For experiment 2, there was an increase in peak latency and a decrease in peak amplitude with decreasing click level; however, the effects of click level were reduced at higher rates. The results indicate that the greater jitter for MLS compared to I-RSA does not substantially affect the dolphin ABR.