Concurrent multi-sample analysis of low expressed biomarkers on single human cells by enzymatically amplified immunodetection in droplets

H. N. Joensson; M. L. Samuels; E. R. Brouzes; M. Medkova; M. Uhlén; H. Andersson Svahn; D. R. Link

Concurrent multi-sample analysis of low expressed biomarkers on single human cells by enzymatically amplified immunodetection in droplets

H. N. Joensson
, M. L. Samuels
, E. R. Brouzes
, M. Medkova
, M. Uhlén
, H. Andersson Svahn
, D. R. Link

Biomedical Engineering

Stony Brook University

KTH Royal Institute of Technology
RainDance Technologies

Research output: Contribution to conference › Paper › peer-review

Abstract

We have developed a novel microfluidic droplet based assay for analysis of low expressed cell surface proteins on individual cells at rates of hundreds of cells/s by antibody coupled enzymatic amplification in monodisperse droplets [1]. Here we expand the method to include concurrent analysis of multiple populations of single cells. We report the validation of the method by analyzing the human monocytic cell line U937 for two low expressed markers, CCR5 and CD19. Comparing our method to standard flow cytometry, we demonstrate increased peak separation, which should allow sorting by these low expressed biomarkers unavailable to flow cytometry.

Original language	English
Pages	1287-1289
Number of pages	3
State	Published - 2008
Event	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States Duration: Oct 12 2008 → Oct 16 2008

Conference

Conference	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
Country/Territory	United States
City	San Diego, CA
Period	10/12/08 → 10/16/08

Keywords

Antibodies
Cell surface biomarkers
Droplets
Enzymes

Cite this

Joensson, H. N., Samuels, M. L., Brouzes, E. R., Medkova, M., Uhlén, M., Andersson Svahn, H., & Link, D. R. (2008). Concurrent multi-sample analysis of low expressed biomarkers on single human cells by enzymatically amplified immunodetection in droplets. 1287-1289. Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.

@conference{e843bf70d7d047a59e2c6839410a763f,

title = "Concurrent multi-sample analysis of low expressed biomarkers on single human cells by enzymatically amplified immunodetection in droplets",

abstract = "We have developed a novel microfluidic droplet based assay for analysis of low expressed cell surface proteins on individual cells at rates of hundreds of cells/s by antibody coupled enzymatic amplification in monodisperse droplets [1]. Here we expand the method to include concurrent analysis of multiple populations of single cells. We report the validation of the method by analyzing the human monocytic cell line U937 for two low expressed markers, CCR5 and CD19. Comparing our method to standard flow cytometry, we demonstrate increased peak separation, which should allow sorting by these low expressed biomarkers unavailable to flow cytometry.",

keywords = "Antibodies, Cell surface biomarkers, Droplets, Enzymes",

author = "Joensson, \{H. N.\} and Samuels, \{M. L.\} and Brouzes, \{E. R.\} and M. Medkova and M. Uhl{\'e}n and \{Andersson Svahn\}, H. and Link, \{D. R.\}",

year = "2008",

language = "English",

pages = "1287--1289",

note = "12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 ; Conference date: 12-10-2008 Through 16-10-2008",

}

Joensson, HN, Samuels, ML, Brouzes, ER, Medkova, M, Uhlén, M, Andersson Svahn, H & Link, DR 2008, 'Concurrent multi-sample analysis of low expressed biomarkers on single human cells by enzymatically amplified immunodetection in droplets', Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States, 10/12/08 - 10/16/08 pp. 1287-1289.

Concurrent multi-sample analysis of low expressed biomarkers on single human cells by enzymatically amplified immunodetection in droplets. / Joensson, H. N.; Samuels, M. L.; Brouzes, E. R. et al.
2008. 1287-1289 Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.

Research output: Contribution to conference › Paper › peer-review

TY - CONF

T1 - Concurrent multi-sample analysis of low expressed biomarkers on single human cells by enzymatically amplified immunodetection in droplets

AU - Joensson, H. N.

AU - Samuels, M. L.

AU - Brouzes, E. R.

AU - Medkova, M.

AU - Uhlén, M.

AU - Andersson Svahn, H.

AU - Link, D. R.

PY - 2008

Y1 - 2008

N2 - We have developed a novel microfluidic droplet based assay for analysis of low expressed cell surface proteins on individual cells at rates of hundreds of cells/s by antibody coupled enzymatic amplification in monodisperse droplets [1]. Here we expand the method to include concurrent analysis of multiple populations of single cells. We report the validation of the method by analyzing the human monocytic cell line U937 for two low expressed markers, CCR5 and CD19. Comparing our method to standard flow cytometry, we demonstrate increased peak separation, which should allow sorting by these low expressed biomarkers unavailable to flow cytometry.

AB - We have developed a novel microfluidic droplet based assay for analysis of low expressed cell surface proteins on individual cells at rates of hundreds of cells/s by antibody coupled enzymatic amplification in monodisperse droplets [1]. Here we expand the method to include concurrent analysis of multiple populations of single cells. We report the validation of the method by analyzing the human monocytic cell line U937 for two low expressed markers, CCR5 and CD19. Comparing our method to standard flow cytometry, we demonstrate increased peak separation, which should allow sorting by these low expressed biomarkers unavailable to flow cytometry.

KW - Antibodies

KW - Cell surface biomarkers

KW - Droplets

KW - Enzymes

UR - https://www.scopus.com/pages/publications/84902507762

M3 - Paper

SP - 1287

EP - 1289

T2 - 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008

Y2 - 12 October 2008 through 16 October 2008

ER -

Joensson HN, Samuels ML, Brouzes ER, Medkova M, Uhlén M, Andersson Svahn H et al.. Concurrent multi-sample analysis of low expressed biomarkers on single human cells by enzymatically amplified immunodetection in droplets. 2008. Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.

Concurrent multi-sample analysis of low expressed biomarkers on single human cells by enzymatically amplified immunodetection in droplets

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