Construction of recombination-deficient strains of Streptococcus gordonii by disruption of the recA gene

M. M. Vickerman; D. G. Heath; D. B. Clewell

doi:10.1128/jb.175.19.6354-6357.1993

Construction of recombination-deficient strains of Streptococcus gordonii by disruption of the recA gene

M. M. Vickerman
, D. G. Heath
, D. B. Clewell

Department of Oral Biology

University at Buffalo

University of Michigan, Ann Arbor

Research output: Contribution to journal › Comment/debate

10 Scopus citations

Abstract

Degenerate oligonucleotide primers were used in a polymerase chain reaction (PCR) to amplify a region of the recA sequence of Streptococcus gordonii Challis. The resulting PCR fragment was cloned into the suicide vector pAM6199 and introduced into strain Challis, giving rise to recombination-deficient strains in which the recA gene was specifically inactivated.

Original language	English
Pages (from-to)	6354-6357
Number of pages	4
Journal	Journal of Bacteriology
Volume	175
Issue number	19
DOIs	https://doi.org/10.1128/jb.175.19.6354-6357.1993
State	Published - 1993

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10.1128/jb.175.19.6354-6357.1993

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title = "Construction of recombination-deficient strains of Streptococcus gordonii by disruption of the recA gene",

abstract = "Degenerate oligonucleotide primers were used in a polymerase chain reaction (PCR) to amplify a region of the recA sequence of Streptococcus gordonii Challis. The resulting PCR fragment was cloned into the suicide vector pAM6199 and introduced into strain Challis, giving rise to recombination-deficient strains in which the recA gene was specifically inactivated.",

author = "Vickerman, \{M. M.\} and Heath, \{D. G.\} and Clewell, \{D. B.\}",

year = "1993",

doi = "10.1128/jb.175.19.6354-6357.1993",

language = "English",

volume = "175",

pages = "6354--6357",

journal = "Journal of Bacteriology",

issn = "0021-9193",

publisher = "American Society for Microbiology",

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TY - JOUR

T1 - Construction of recombination-deficient strains of Streptococcus gordonii by disruption of the recA gene

AU - Vickerman, M. M.

AU - Heath, D. G.

AU - Clewell, D. B.

PY - 1993

Y1 - 1993

N2 - Degenerate oligonucleotide primers were used in a polymerase chain reaction (PCR) to amplify a region of the recA sequence of Streptococcus gordonii Challis. The resulting PCR fragment was cloned into the suicide vector pAM6199 and introduced into strain Challis, giving rise to recombination-deficient strains in which the recA gene was specifically inactivated.

AB - Degenerate oligonucleotide primers were used in a polymerase chain reaction (PCR) to amplify a region of the recA sequence of Streptococcus gordonii Challis. The resulting PCR fragment was cloned into the suicide vector pAM6199 and introduced into strain Challis, giving rise to recombination-deficient strains in which the recA gene was specifically inactivated.

UR - https://www.scopus.com/pages/publications/0027442892

U2 - 10.1128/jb.175.19.6354-6357.1993

DO - 10.1128/jb.175.19.6354-6357.1993

M3 - Comment/debate

C2 - 8407809

SN - 0021-9193

VL - 175

SP - 6354

EP - 6357

JO - Journal of Bacteriology

JF - Journal of Bacteriology

IS - 19

ER -

Construction of recombination-deficient strains of Streptococcus gordonii by disruption of the recA gene

Abstract

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