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Correction to: Compartment-specific labeling of bacterial periplasmic proteins by peroxidase-mediated biotinylation (ACS Infectious Diseases (2018) 4:6 (918-925) DOI: 10.1021/acsinfecdis.8b00044)

Research output: Contribution to journalComment/debate

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Abstract

In the original published version, the Methods section cited the incorrect AddGene reference number for the template plasmid used to clone the APEX gene and provided incorrect purification conditions for the tyramide reagents. Under Cloning, the methods should read: "N-terminal V5-tagged APEX was subcloned from pcDNA3- mito-APEX (Addgene plasmid # 42607)." Under Synthesis of Tyramide Azide and Tyramide Alkyne, the purification conditions should read: "The residue was dissolved in acetonitrile and purified by high-pressure liquid chromatography (HPLC) (20-50% acetonitrile in water with 1% trifluoroacetic acid over 85 min, retention time = 26 min, 35% acetonitrile)."

Original languageEnglish
Pages (from-to)1157
Number of pages1
JournalACS Infectious Diseases
Volume4
Issue number7
DOIs
StatePublished - Jul 13 2018

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