Crayfish and Drosophila NMJs

Many synaptic studies have utilized the experimental advantages of the Arthropod NMJ and the most prominent preparations have been the crayfish and Drosophila larval NMJs. Early cellular studies in the crayfish established the framework for later molecular studies in Drosophila. The two neuromuscular systems are compared including the advantages presented by each preparation for cellular analysis. Beginning with the early work in the crayfish, research developments are followed in the areas of structure/function relationships, activity-dependent synaptic plasticity/development and synaptic homeostasis. A reoccurring theme in these studies is the regulation of active zone structure and function. Early studies in the crayfish focused on the role of active zone number/size and possible functional heterogeneity in regulating transmitter release. Recent studies in Drosophila have begun to characterize this heterogeneity using new approaches that combine imaging of transmitter release, Ca²⁺ influx and molecular composition for individual active zones.