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Cross-ancestry atlas of gene, isoform, and splicing regulation in the developing human brain

  • PsychENCODE Consortium
  • University of California at Los Angeles
  • SUNY Upstate Medical University
  • Gladstone Institutes
  • University of Pennsylvania
  • Children's Hospital of Philadelphia
  • University of North Carolina at Chapel Hill
  • Université de Paris
  • Yale University
  • University of Massachusetts Medical School
  • Sage Bionetworks
  • Harvard University
  • McLean Hospital
  • Broad Institute
  • Lieber Institute for Brain Development
  • Johns Hopkins University
  • Neumora Therapeutics, Inc.
  • Cardiff University
  • Carnegie Mellon University
  • Dana-Farber Cancer Institute
  • Brigham and Women’s Hospital
  • University of California at San Francisco
  • Chan Zuckerberg Biohub
  • Central South University
  • University of Pennsylvania
  • Research Institute
  • Université Paris Cité
  • Harvard University
  • Yale University

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

Neuropsychiatric genome-wide association studies (GWASs), including those for autism spectrum disorder and schizophrenia, show strong enrichment for regulatory elements in the developing brain. However, prioritizing risk genes and mechanisms is challenging without a unified regulatory atlas. Across 672 diverse developing human brains, we identified 15,752 genes harboring gene, isoform, and/or splicing quantitative trait loci, mapping 3739 to cellular contexts. Gene expression heritability drops during development, likely reflecting both increasing cellular heterogeneity and the intrinsic properties of neuronal maturation. Isoform-level regulation, particularly in the second trimester, mediated the largest proportion of GWAS heritability. Through colocalization, we prioritized mechanisms for about 60% of GWAS loci across five disorders, exceeding adult brain findings. Finally, we contextualized results within gene and isoform coexpression networks, revealing the comprehensive landscape of transcriptome regulation in development and disease.

Original languageEnglish
Article numbereadh0829
JournalScience
Volume384
Issue number6698
DOIs
StatePublished - May 24 2024

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