Crystallization and preliminary diffraction analysis of cholesterol esterase from Candida cylindracea

Cholesterol esterase (EC 3.1.1.13) from the microorganism Candida cylindracea has been crystallized in two forms. Crystals, typically 0.30 × 0.15 × 0.10 mm in size, diffract rotating anode generated x-ray to beyond 3 Å are suitable for data collection for an x-ray crystallographic investigation. A monoclinic crystal form in the space group P2₁ was found to have cell dimensions of a = 122.9 A ̊, b = 101.0 A ̊, c = 95.2 A ̊ and β = 108.3°. The asymmetric unit of cell contains two dimers of 129 kDa each. A second crystal form, in th triclinic space group P1, has cell dimensions of a = A ̊, b = 88.7 A ̊, c = 58.6 A ̊, α = 93.3°, β = 113.8° and γ = 96.0°, and has one dimer per asymmetric unit.