CYP1A1 and MT1K are congener specific biomarker genes for liver diseases induced by PCBs

Sisir K. Dutta; Somiranjan Ghosh; Supriyo De; Eric P. Hoffman

doi:10.1016/j.etap.2007.10.018

CYP1A1 and MT1K are congener specific biomarker genes for liver diseases induced by PCBs

Sisir K. Dutta
, Somiranjan Ghosh
, Supriyo De
, Eric P. Hoffman

Pharmaceutical Sciences

Binghamton University

Howard University

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

We have identified two most significant biomarker genes, CYP1A1 (69.81 up-regulation) and MT1K (14.66 up-regulation), showing highest overexpression at p-value <0.005. These were selected out of several hundred genes induced in vitro, using PCB exposed human liver (HepG2) cells. Over expression of the CYP1A1 (cytochrome P450) gene was specific to PCB-77 and MT1K (Metallothionein) to PCB-153. Affymetrix oligonucleotide microarrays (mRNA) were used to screen the entire genome of human liver cells in a time-dependent exposure and were further validated by quantitative real-time RT-PCR.

Original language	English
Pages (from-to)	218-221
Number of pages	4
Journal	Environmental Toxicology and Pharmacology
Volume	25
Issue number	2
DOIs	https://doi.org/10.1016/j.etap.2007.10.018
State	Published - Mar 2008

Keywords

Biomarker genes
CYP1A1
MT1K
PCB-153
PCB-77

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10.1016/j.etap.2007.10.018

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title = "CYP1A1 and MT1K are congener specific biomarker genes for liver diseases induced by PCBs",

abstract = "We have identified two most significant biomarker genes, CYP1A1 (69.81 up-regulation) and MT1K (14.66 up-regulation), showing highest overexpression at p-value <0.005. These were selected out of several hundred genes induced in vitro, using PCB exposed human liver (HepG2) cells. Over expression of the CYP1A1 (cytochrome P450) gene was specific to PCB-77 and MT1K (Metallothionein) to PCB-153. Affymetrix oligonucleotide microarrays (mRNA) were used to screen the entire genome of human liver cells in a time-dependent exposure and were further validated by quantitative real-time RT-PCR.",

keywords = "Biomarker genes, CYP1A1, MT1K, PCB-153, PCB-77",

author = "Dutta, \{Sisir K.\} and Somiranjan Ghosh and Supriyo De and Hoffman, \{Eric P.\}",

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AU - Ghosh, Somiranjan

AU - De, Supriyo

AU - Hoffman, Eric P.

PY - 2008/3

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AB - We have identified two most significant biomarker genes, CYP1A1 (69.81 up-regulation) and MT1K (14.66 up-regulation), showing highest overexpression at p-value <0.005. These were selected out of several hundred genes induced in vitro, using PCB exposed human liver (HepG2) cells. Over expression of the CYP1A1 (cytochrome P450) gene was specific to PCB-77 and MT1K (Metallothionein) to PCB-153. Affymetrix oligonucleotide microarrays (mRNA) were used to screen the entire genome of human liver cells in a time-dependent exposure and were further validated by quantitative real-time RT-PCR.

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KW - MT1K

KW - PCB-153

KW - PCB-77

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DO - 10.1016/j.etap.2007.10.018

M3 - Article

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JO - Environmental Toxicology and Pharmacology

JF - Environmental Toxicology and Pharmacology

IS - 2

ER -

CYP1A1 and MT1K are congener specific biomarker genes for liver diseases induced by PCBs

Abstract

Keywords

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