Design and synthesis of fluorescence-labeled nucleotide with a cleavable azo linker for DNA sequencing

Lianjiang Tan; Yazhi Liu; Qinglai Yang; Xiaowei Li; Xin Yan Wu; Bing Gong; Yu Mei Shen; Zhifeng Shao

doi:10.1039/c5cc09131d

Design and synthesis of fluorescence-labeled nucleotide with a cleavable azo linker for DNA sequencing

Lianjiang Tan
, Yazhi Liu
, Qinglai Yang
, Xiaowei Li
, Xin Yan Wu
, Bing Gong
, Yu Mei Shen
, Zhifeng Shao

Chemistry

University at Buffalo

Shanghai Jiao Tong University
East China University of Science and Technology

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

A cleavable azo linker was synthesized and reacted with 5-(6)-carboxytetramethyl rhodamine succinimidyl ester, followed by further reactions with di(N-succinimidyl) carbonate and 5-(3-amino-1-propynyl)-2′-deoxyuridine 5′-triphosphate [dUTP(AP₃)] to obtain the terminal product dUTP-azo linker-TAMRA as a potential reversible terminator for DNA sequencing by synthesis with no need for 3′-OH blocking.

Original language	English
Pages (from-to)	954-957
Number of pages	4
Journal	Chemical Communications
Volume	52
Issue number	5
DOIs	https://doi.org/10.1039/c5cc09131d
State	Published - 2016

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title = "Design and synthesis of fluorescence-labeled nucleotide with a cleavable azo linker for DNA sequencing",

abstract = "A cleavable azo linker was synthesized and reacted with 5-(6)-carboxytetramethyl rhodamine succinimidyl ester, followed by further reactions with di(N-succinimidyl) carbonate and 5-(3-amino-1-propynyl)-2′-deoxyuridine 5′-triphosphate [dUTP(AP3)] to obtain the terminal product dUTP-azo linker-TAMRA as a potential reversible terminator for DNA sequencing by synthesis with no need for 3′-OH blocking.",

author = "Lianjiang Tan and Yazhi Liu and Qinglai Yang and Xiaowei Li and Wu, \{Xin Yan\} and Bing Gong and Shen, \{Yu Mei\} and Zhifeng Shao",

note = "Publisher Copyright: {\textcopyright} The Royal Society of Chemistry 2016.",

year = "2016",

doi = "10.1039/c5cc09131d",

language = "English",

volume = "52",

pages = "954--957",

journal = "Chemical Communications",

issn = "1359-7345",

publisher = "Royal Society of Chemistry",

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TY - JOUR

T1 - Design and synthesis of fluorescence-labeled nucleotide with a cleavable azo linker for DNA sequencing

AU - Tan, Lianjiang

AU - Liu, Yazhi

AU - Yang, Qinglai

AU - Li, Xiaowei

AU - Wu, Xin Yan

AU - Gong, Bing

AU - Shen, Yu Mei

AU - Shao, Zhifeng

PY - 2016

Y1 - 2016

N2 - A cleavable azo linker was synthesized and reacted with 5-(6)-carboxytetramethyl rhodamine succinimidyl ester, followed by further reactions with di(N-succinimidyl) carbonate and 5-(3-amino-1-propynyl)-2′-deoxyuridine 5′-triphosphate [dUTP(AP3)] to obtain the terminal product dUTP-azo linker-TAMRA as a potential reversible terminator for DNA sequencing by synthesis with no need for 3′-OH blocking.

AB - A cleavable azo linker was synthesized and reacted with 5-(6)-carboxytetramethyl rhodamine succinimidyl ester, followed by further reactions with di(N-succinimidyl) carbonate and 5-(3-amino-1-propynyl)-2′-deoxyuridine 5′-triphosphate [dUTP(AP3)] to obtain the terminal product dUTP-azo linker-TAMRA as a potential reversible terminator for DNA sequencing by synthesis with no need for 3′-OH blocking.

UR - https://www.scopus.com/pages/publications/84954071012

U2 - 10.1039/c5cc09131d

DO - 10.1039/c5cc09131d

M3 - Article

C2 - 26587573

SN - 1359-7345

VL - 52

SP - 954

EP - 957

JO - Chemical Communications

JF - Chemical Communications

IS - 5

ER -

Design and synthesis of fluorescence-labeled nucleotide with a cleavable azo linker for DNA sequencing

Abstract

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