Detailed sterol compositions of two pathogenic rust fungi

Teliospores of cedar-apple rust Gymnosporangium juniperi-virginianae were collected from the eastern red cedar Juniperus virginiana, and aeciospores of quince rust G. clavipes were collected from the fruit of English hawthorn Crataegus laevigata. The sterol fractions were separated by HPLC, and their identities were determined by 600 MHz ¹H NMR. Twenty-six sterols were isolated from G. juniperi-virginianae and 18 sterols were isolated from G. clavipes. The principal sterol of both fungi was (Z)-stigmasta-7,24(28)-dien- 3β-ol. Other major sterols were (24S)-ergost-7-en-3β-ol, (24S)-stigmast-7-en-3β-ol, and (24S)-stigmasta-5,7-dien-3β-ol. The sterols of the hosts were found to be very different from those of the fungi. The 24-alkyl sterols of the fungi had the 24α-configuration, whereas those of the hosts had the 24β-configuration. Similarities to the sterol composition of the AIDS pneumonia fungus Pneumocystis carinii are discussed.