Di-O-alkylglycerol, mono-O-alkylglycerol and ceramide inositol phosphates of Leishmania Mexicana Mexicana promastigotes

B. N. Singh; C. E. Costello; D. H. Beach; G. G. Holz

doi:10.1016/S0006-291X(88)81007-6

Di-O-alkylglycerol, mono-O-alkylglycerol and ceramide inositol phosphates of Leishmania Mexicana Mexicana promastigotes

B. N. Singh
, C. E. Costello
, D. H. Beach
, G. G. Holz

Medicine

Upstate Medical University

SUNY Upstate Medical University
Massachusetts Institute of Technology

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

Three acidic unsaponifiable lipid fractions were isolated by chromatographic methods from sandfly vector stages (promastigotes) of a protozoan parasite of man, Leishmania mexicana mexicana, cultured in vitro. Fast atom bombardment mass spectrometry, fast atom bombardment collision induced tandem mass spectrometry and metabolic labeling were used to characterize these lipids as di-O-alkylphosphatidyl-inositols, lyso-1-O-alkylphosphatidylinositols and inositol phosphosphingolipids. Molecular species of the dialkyl forms, new to natural product biochemistry, had a 20:0 substituent and either 17:1 or 18:1. The monoalkyl forms had either 17:0 or 18:0. The predominant ceramide had the 16:1 base and the lesser component the 16:0 base. In both, the N-acyl group was 18:0.

Original language	English
Pages (from-to)	1239-1246
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	157
Issue number	3
DOIs	https://doi.org/10.1016/S0006-291X(88)81007-6
State	Published - Dec 30 1988

Access to Document

10.1016/S0006-291X(88)81007-6

Cite this

@article{7d6c192a30854c59bf8d84238a039784,

title = "Di-O-alkylglycerol, mono-O-alkylglycerol and ceramide inositol phosphates of Leishmania Mexicana Mexicana promastigotes",

abstract = "Three acidic unsaponifiable lipid fractions were isolated by chromatographic methods from sandfly vector stages (promastigotes) of a protozoan parasite of man, Leishmania mexicana mexicana, cultured in vitro. Fast atom bombardment mass spectrometry, fast atom bombardment collision induced tandem mass spectrometry and metabolic labeling were used to characterize these lipids as di-O-alkylphosphatidyl-inositols, lyso-1-O-alkylphosphatidylinositols and inositol phosphosphingolipids. Molecular species of the dialkyl forms, new to natural product biochemistry, had a 20:0 substituent and either 17:1 or 18:1. The monoalkyl forms had either 17:0 or 18:0. The predominant ceramide had the 16:1 base and the lesser component the 16:0 base. In both, the N-acyl group was 18:0.",

author = "Singh, \{B. N.\} and Costello, \{C. E.\} and Beach, \{D. H.\} and Holz, \{G. G.\}",

year = "1988",

month = dec,

day = "30",

doi = "10.1016/S0006-291X(88)81007-6",

language = "English",

volume = "157",

pages = "1239--1246",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

number = "3",

}

TY - JOUR

T1 - Di-O-alkylglycerol, mono-O-alkylglycerol and ceramide inositol phosphates of Leishmania Mexicana Mexicana promastigotes

AU - Singh, B. N.

AU - Costello, C. E.

AU - Beach, D. H.

AU - Holz, G. G.

PY - 1988/12/30

Y1 - 1988/12/30

N2 - Three acidic unsaponifiable lipid fractions were isolated by chromatographic methods from sandfly vector stages (promastigotes) of a protozoan parasite of man, Leishmania mexicana mexicana, cultured in vitro. Fast atom bombardment mass spectrometry, fast atom bombardment collision induced tandem mass spectrometry and metabolic labeling were used to characterize these lipids as di-O-alkylphosphatidyl-inositols, lyso-1-O-alkylphosphatidylinositols and inositol phosphosphingolipids. Molecular species of the dialkyl forms, new to natural product biochemistry, had a 20:0 substituent and either 17:1 or 18:1. The monoalkyl forms had either 17:0 or 18:0. The predominant ceramide had the 16:1 base and the lesser component the 16:0 base. In both, the N-acyl group was 18:0.

AB - Three acidic unsaponifiable lipid fractions were isolated by chromatographic methods from sandfly vector stages (promastigotes) of a protozoan parasite of man, Leishmania mexicana mexicana, cultured in vitro. Fast atom bombardment mass spectrometry, fast atom bombardment collision induced tandem mass spectrometry and metabolic labeling were used to characterize these lipids as di-O-alkylphosphatidyl-inositols, lyso-1-O-alkylphosphatidylinositols and inositol phosphosphingolipids. Molecular species of the dialkyl forms, new to natural product biochemistry, had a 20:0 substituent and either 17:1 or 18:1. The monoalkyl forms had either 17:0 or 18:0. The predominant ceramide had the 16:1 base and the lesser component the 16:0 base. In both, the N-acyl group was 18:0.

UR - https://www.scopus.com/pages/publications/0024203634

U2 - 10.1016/S0006-291X(88)81007-6

DO - 10.1016/S0006-291X(88)81007-6

M3 - Article

C2 - 3207423

SN - 0006-291X

VL - 157

SP - 1239

EP - 1246

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3

ER -

Di-O-alkylglycerol, mono-O-alkylglycerol and ceramide inositol phosphates of Leishmania Mexicana Mexicana promastigotes

Abstract

Access to Document

Other files and links

Fingerprint

Cite this