Differential actions and excitotoxicity of glutamate agonists on motoneurons in adult mouse cervical spinal cord slices

N. Hori; Y. Tan; M. King; N. L. Strominger; D. O. Carpenter

doi:10.1016/S0006-8993(02)03538-2

Differential actions and excitotoxicity of glutamate agonists on motoneurons in adult mouse cervical spinal cord slices

N. Hori
, Y. Tan
, M. King
, N. L. Strominger
, D. O. Carpenter

Environmental Health Sciences

University at Albany

SUNY Albany
Albany Medical College

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Electrical activity was recorded from motoneurons in adult mouse cervical spinal cord (C4-8) slices. Motoneurons showed almost no response to ionophoretic application of N-methyl-D-aspartic acid (NMDA) in both control and Mg²⁺-free media, but very sensitive to kainate (KA) and amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA). Bath perfusion of KA, AMPA or glucose/O₂ free Krebs-Ringer solution, but not bath perfusion of NMDA, caused membrane depolarization within 3 min and beading of the dendrite trees after more than 10 min perfusion. Our results indicate that adult motoneurons have few or no NMDA receptors.

Original language	English
Pages (from-to)	434-438
Number of pages	5
Journal	Brain Research
Volume	958
Issue number	2
DOIs	https://doi.org/10.1016/S0006-8993(02)03538-2
State	Published - Dec 27 2002

Keywords

AMPA
Cell death
Excitatory amino acids: anatomy and physiology
Glutamate
Ischemia
Kainate
NMDA
Neurotransmitters, modulators, transporters, and receptors

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10.1016/S0006-8993(02)03538-2

Cite this

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title = "Differential actions and excitotoxicity of glutamate agonists on motoneurons in adult mouse cervical spinal cord slices",

abstract = "Electrical activity was recorded from motoneurons in adult mouse cervical spinal cord (C4-8) slices. Motoneurons showed almost no response to ionophoretic application of N-methyl-D-aspartic acid (NMDA) in both control and Mg2+-free media, but very sensitive to kainate (KA) and amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA). Bath perfusion of KA, AMPA or glucose/O2 free Krebs-Ringer solution, but not bath perfusion of NMDA, caused membrane depolarization within 3 min and beading of the dendrite trees after more than 10 min perfusion. Our results indicate that adult motoneurons have few or no NMDA receptors.",

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doi = "10.1016/S0006-8993(02)03538-2",

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T1 - Differential actions and excitotoxicity of glutamate agonists on motoneurons in adult mouse cervical spinal cord slices

AU - Hori, N.

AU - Tan, Y.

AU - King, M.

AU - Strominger, N. L.

AU - Carpenter, D. O.

PY - 2002/12/27

Y1 - 2002/12/27

N2 - Electrical activity was recorded from motoneurons in adult mouse cervical spinal cord (C4-8) slices. Motoneurons showed almost no response to ionophoretic application of N-methyl-D-aspartic acid (NMDA) in both control and Mg2+-free media, but very sensitive to kainate (KA) and amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA). Bath perfusion of KA, AMPA or glucose/O2 free Krebs-Ringer solution, but not bath perfusion of NMDA, caused membrane depolarization within 3 min and beading of the dendrite trees after more than 10 min perfusion. Our results indicate that adult motoneurons have few or no NMDA receptors.

AB - Electrical activity was recorded from motoneurons in adult mouse cervical spinal cord (C4-8) slices. Motoneurons showed almost no response to ionophoretic application of N-methyl-D-aspartic acid (NMDA) in both control and Mg2+-free media, but very sensitive to kainate (KA) and amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA). Bath perfusion of KA, AMPA or glucose/O2 free Krebs-Ringer solution, but not bath perfusion of NMDA, caused membrane depolarization within 3 min and beading of the dendrite trees after more than 10 min perfusion. Our results indicate that adult motoneurons have few or no NMDA receptors.

KW - AMPA

KW - Cell death

KW - Excitatory amino acids: anatomy and physiology

KW - Glutamate

KW - Ischemia

KW - Kainate

KW - NMDA

KW - Neurotransmitters, modulators, transporters, and receptors

UR - https://www.scopus.com/pages/publications/0037184699

U2 - 10.1016/S0006-8993(02)03538-2

DO - 10.1016/S0006-8993(02)03538-2

M3 - Article

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SN - 0006-8993

VL - 958

SP - 434

EP - 438

JO - Brain Research

JF - Brain Research

IS - 2

ER -

Differential actions and excitotoxicity of glutamate agonists on motoneurons in adult mouse cervical spinal cord slices

Abstract

Keywords

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