DNA nanoswitches: A quantitative platform for gel-based biomolecular interaction analysis

Mounir A. Koussa; Ken Halvorsen; Andrew Ward; Wesley P. Wong

doi:10.1038/nmeth.3209

DNA nanoswitches: A quantitative platform for gel-based biomolecular interaction analysis

Mounir A. Koussa
, Ken Halvorsen
, Andrew Ward
, Wesley P. Wong

Institute for RNA Science and Technology

University at Albany

Harvard University
Boston Children's Hospital

Research output: Contribution to journal › Article › peer-review

68 Scopus citations

Abstract

We introduce a nanoscale experimental platform that enables kinetic and equilibrium measurements of a wide range of molecular interactions using a gel electrophoresis readout. Programmable, self-assembled DNA nanoswitches serve both as templates for positioning molecules and as sensitive, quantitative reporters of molecular association and dissociation. We demonstrated this low-cost, versatile, 'lab-on-a-molecule' system by characterizing ten different interactions, including a complex four-body interaction with five discernible states.

Original language	English
Pages (from-to)	123-126
Number of pages	4
Journal	Nature Methods
Volume	12
Issue number	2
DOIs	https://doi.org/10.1038/nmeth.3209
State	Published - Jan 1 2015

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abstract = "We introduce a nanoscale experimental platform that enables kinetic and equilibrium measurements of a wide range of molecular interactions using a gel electrophoresis readout. Programmable, self-assembled DNA nanoswitches serve both as templates for positioning molecules and as sensitive, quantitative reporters of molecular association and dissociation. We demonstrated this low-cost, versatile, 'lab-on-a-molecule' system by characterizing ten different interactions, including a complex four-body interaction with five discernible states.",

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AU - Wong, Wesley P.

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AB - We introduce a nanoscale experimental platform that enables kinetic and equilibrium measurements of a wide range of molecular interactions using a gel electrophoresis readout. Programmable, self-assembled DNA nanoswitches serve both as templates for positioning molecules and as sensitive, quantitative reporters of molecular association and dissociation. We demonstrated this low-cost, versatile, 'lab-on-a-molecule' system by characterizing ten different interactions, including a complex four-body interaction with five discernible states.

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DNA nanoswitches: A quantitative platform for gel-based biomolecular interaction analysis

Abstract

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