Effects of inositol trisphosphate on calcium mobilization in bone cells

Rebecca Falsafi; Dimitris N. Tatakis; Susan Hagel-Bradway; Rosemary Dziak

doi:10.1007/BF02556256

Effects of inositol trisphosphate on calcium mobilization in bone cells

Rebecca Falsafi
, Dimitris N. Tatakis
, Susan Hagel-Bradway
, Rosemary Dziak

Department of Oral Biology

University at Buffalo

SUNY Buffalo

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

The effect of inositol 1,4,5 trisphosphate (IP₃) on calcium mobilization was studied in human osteosarcoma lines, Saos-2 and G292, as well as isolated rat osteoblastic and osteoclastic cells. Cells were permeabilized with saponin and calcium mobilization was studied with the fluorescent dye, fura-2 in a recording spectrofluorometer. IP₃ (10 μM) increased calcium release in all cell types studied. The effect was dependent on ATP and occurred in the presence of mitochondrial inhibitors. The effect was not seen with inositol 1-phosphate (IP) or inositol 1,4-diphosphate (IP₂). Inositol 1,3,4,5 tetrakisphosphate (IP₄) appeared to elicit a decrease in the calcium released. Depletion of the intracellular pool with the calcium ionophore, ionomycin, as well as incubation with the inhibitor of intracellular calcium mobilization, TMB-8, obliterated the IP₃ effect. The results are consistent with the hypothesis that increases in IP₃ can cause a rapid elevation of bone cell cytosolic calcium.

Original language	English
Pages (from-to)	333-339
Number of pages	7
Journal	Calcified tissue international
Volume	49
Issue number	5
DOIs	https://doi.org/10.1007/BF02556256
State	Published - Sep 1991

Keywords

Bone cells
Calcium
Inositol 1,4,5 trisphosphate
Osteoblasts

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10.1007/BF02556256

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@article{589ef4fee495451184bc700cae3e3d08,

title = "Effects of inositol trisphosphate on calcium mobilization in bone cells",

abstract = "The effect of inositol 1,4,5 trisphosphate (IP3) on calcium mobilization was studied in human osteosarcoma lines, Saos-2 and G292, as well as isolated rat osteoblastic and osteoclastic cells. Cells were permeabilized with saponin and calcium mobilization was studied with the fluorescent dye, fura-2 in a recording spectrofluorometer. IP3 (10 μM) increased calcium release in all cell types studied. The effect was dependent on ATP and occurred in the presence of mitochondrial inhibitors. The effect was not seen with inositol 1-phosphate (IP) or inositol 1,4-diphosphate (IP2). Inositol 1,3,4,5 tetrakisphosphate (IP4) appeared to elicit a decrease in the calcium released. Depletion of the intracellular pool with the calcium ionophore, ionomycin, as well as incubation with the inhibitor of intracellular calcium mobilization, TMB-8, obliterated the IP3 effect. The results are consistent with the hypothesis that increases in IP3 can cause a rapid elevation of bone cell cytosolic calcium.",

keywords = "Bone cells, Calcium, Inositol 1,4,5 trisphosphate, Osteoblasts",

author = "Rebecca Falsafi and Tatakis, \{Dimitris N.\} and Susan Hagel-Bradway and Rosemary Dziak",

year = "1991",

month = sep,

doi = "10.1007/BF02556256",

language = "English",

volume = "49",

pages = "333--339",

journal = "Calcified tissue international",

issn = "0171-967X",

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TY - JOUR

T1 - Effects of inositol trisphosphate on calcium mobilization in bone cells

AU - Falsafi, Rebecca

AU - Tatakis, Dimitris N.

AU - Hagel-Bradway, Susan

AU - Dziak, Rosemary

PY - 1991/9

Y1 - 1991/9

N2 - The effect of inositol 1,4,5 trisphosphate (IP3) on calcium mobilization was studied in human osteosarcoma lines, Saos-2 and G292, as well as isolated rat osteoblastic and osteoclastic cells. Cells were permeabilized with saponin and calcium mobilization was studied with the fluorescent dye, fura-2 in a recording spectrofluorometer. IP3 (10 μM) increased calcium release in all cell types studied. The effect was dependent on ATP and occurred in the presence of mitochondrial inhibitors. The effect was not seen with inositol 1-phosphate (IP) or inositol 1,4-diphosphate (IP2). Inositol 1,3,4,5 tetrakisphosphate (IP4) appeared to elicit a decrease in the calcium released. Depletion of the intracellular pool with the calcium ionophore, ionomycin, as well as incubation with the inhibitor of intracellular calcium mobilization, TMB-8, obliterated the IP3 effect. The results are consistent with the hypothesis that increases in IP3 can cause a rapid elevation of bone cell cytosolic calcium.

AB - The effect of inositol 1,4,5 trisphosphate (IP3) on calcium mobilization was studied in human osteosarcoma lines, Saos-2 and G292, as well as isolated rat osteoblastic and osteoclastic cells. Cells were permeabilized with saponin and calcium mobilization was studied with the fluorescent dye, fura-2 in a recording spectrofluorometer. IP3 (10 μM) increased calcium release in all cell types studied. The effect was dependent on ATP and occurred in the presence of mitochondrial inhibitors. The effect was not seen with inositol 1-phosphate (IP) or inositol 1,4-diphosphate (IP2). Inositol 1,3,4,5 tetrakisphosphate (IP4) appeared to elicit a decrease in the calcium released. Depletion of the intracellular pool with the calcium ionophore, ionomycin, as well as incubation with the inhibitor of intracellular calcium mobilization, TMB-8, obliterated the IP3 effect. The results are consistent with the hypothesis that increases in IP3 can cause a rapid elevation of bone cell cytosolic calcium.

KW - Bone cells

KW - Calcium

KW - Inositol 1,4,5 trisphosphate

KW - Osteoblasts

UR - https://www.scopus.com/pages/publications/0026331187

U2 - 10.1007/BF02556256

DO - 10.1007/BF02556256

M3 - Article

C2 - 1782574

SN - 0171-967X

VL - 49

SP - 333

EP - 339

JO - Calcified tissue international

JF - Calcified tissue international

IS - 5

ER -

Effects of inositol trisphosphate on calcium mobilization in bone cells

Abstract

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