@inproceedings{4fea6f1c11364a5ea9a283c2dc757c91,
title = "Fluorescence lifetime imaging and its applications in cellular microenvironment measurement and auxiliary diagnosis",
abstract = "We present our recent work on the applications of fluorescence lifetime imaging microscopy(FLIM), including the monitoring of macromolecule dynamic changes in the nucleolar compartments and the auxiliary diagnosis of H and E-stained sections. We demonstrated the capability of FLIM to measure protein concentration in the specific cellular compartments in live cells. We proposed to use FLIM to monitor changes in intracellular protein concentration caused by various factors e.g. cell cycle progression, drug treatment etc. In the future, FLIM technology is expected to be combined with super-resolution optical imaging. FLIM with molecular resolution will have the potential to serve as a powerful tool for discovering new phenomena and revealing new mechanisms in biomedical research, which will effectively promote the development of life science.",
keywords = "FLIM, H\&E, diagnosis, macromolecule, nucleus",
author = "Teng Luo and Levchenko, \{Svitlana M.\} and Artem Pliss and Xiao Peng and Wei Yan and Prasad, \{Paras N.\} and Liwei Liu and Junle Qu",
note = "Publisher Copyright: {\textcopyright} 2018 SPIE.; Biophotonics and Immune Responses XIII 2018 ; Conference date: 29-01-2018 Through 29-01-2018",
year = "2018",
doi = "10.1117/12.2289017",
language = "English",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
publisher = "SPIE",
editor = "Chen, \{Wei R.\}",
booktitle = "Biophotonics and Immune Responses XIII",
address = "United States",
}