Fluoride stimulates [3H]thymidine incorporation and alkaline phosphatase production by human osteoblasts

M. A. Khokher; P. Dandona

doi:10.1016/0026-0495(90)90081-M

Fluoride stimulates [³H]thymidine incorporation and alkaline phosphatase production by human osteoblasts

M. A. Khokher
, P. Dandona

Medicine

University at Buffalo

Research output: Contribution to journal › Article › peer-review

59 Scopus citations

Abstract

The effect of sodium fluoride on alkaline phosphatase (ALP) release and [³H]thymidine uptake by human osteoblasts in culture was investigated. Sodium fluoride stimulated both ALP release and [³H]thymidine uptake at concentrations of sodium fluoride greater than 250 μmol/L. This stimulation was similar in magnitude to that induced by 1,25-dihydroxycholecalciferol. The fluoride-induced increase in ALP was inhibited by verapamil, a calcium channel blocker. We conclude that sodium fluoride stimulates osteoblasts to proliferate and to release ALP. This stimulation by fluoride is dependent on calcium influx. Fluoride-induced stimulation of human osteoblasts may be relevant to its effect in enhancing bone formation in patients with osteoporosis.

Original language	English
Pages (from-to)	1118-1121
Number of pages	4
Journal	Metabolism: Clinical and Experimental
Volume	39
Issue number	11
DOIs	https://doi.org/10.1016/0026-0495(90)90081-M
State	Published - Nov 1990

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title = "Fluoride stimulates [3H]thymidine incorporation and alkaline phosphatase production by human osteoblasts",

abstract = "The effect of sodium fluoride on alkaline phosphatase (ALP) release and [3H]thymidine uptake by human osteoblasts in culture was investigated. Sodium fluoride stimulated both ALP release and [3H]thymidine uptake at concentrations of sodium fluoride greater than 250 μmol/L. This stimulation was similar in magnitude to that induced by 1,25-dihydroxycholecalciferol. The fluoride-induced increase in ALP was inhibited by verapamil, a calcium channel blocker. We conclude that sodium fluoride stimulates osteoblasts to proliferate and to release ALP. This stimulation by fluoride is dependent on calcium influx. Fluoride-induced stimulation of human osteoblasts may be relevant to its effect in enhancing bone formation in patients with osteoporosis.",

author = "Khokher, \{M. A.\} and P. Dandona",

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T1 - Fluoride stimulates [3H]thymidine incorporation and alkaline phosphatase production by human osteoblasts

AU - Khokher, M. A.

AU - Dandona, P.

PY - 1990/11

Y1 - 1990/11

N2 - The effect of sodium fluoride on alkaline phosphatase (ALP) release and [3H]thymidine uptake by human osteoblasts in culture was investigated. Sodium fluoride stimulated both ALP release and [3H]thymidine uptake at concentrations of sodium fluoride greater than 250 μmol/L. This stimulation was similar in magnitude to that induced by 1,25-dihydroxycholecalciferol. The fluoride-induced increase in ALP was inhibited by verapamil, a calcium channel blocker. We conclude that sodium fluoride stimulates osteoblasts to proliferate and to release ALP. This stimulation by fluoride is dependent on calcium influx. Fluoride-induced stimulation of human osteoblasts may be relevant to its effect in enhancing bone formation in patients with osteoporosis.

AB - The effect of sodium fluoride on alkaline phosphatase (ALP) release and [3H]thymidine uptake by human osteoblasts in culture was investigated. Sodium fluoride stimulated both ALP release and [3H]thymidine uptake at concentrations of sodium fluoride greater than 250 μmol/L. This stimulation was similar in magnitude to that induced by 1,25-dihydroxycholecalciferol. The fluoride-induced increase in ALP was inhibited by verapamil, a calcium channel blocker. We conclude that sodium fluoride stimulates osteoblasts to proliferate and to release ALP. This stimulation by fluoride is dependent on calcium influx. Fluoride-induced stimulation of human osteoblasts may be relevant to its effect in enhancing bone formation in patients with osteoporosis.

UR - https://www.scopus.com/pages/publications/0025514281

U2 - 10.1016/0026-0495(90)90081-M

DO - 10.1016/0026-0495(90)90081-M

M3 - Article

C2 - 2233270

SN - 0026-0495

VL - 39

SP - 1118

EP - 1121

JO - Metabolism: Clinical and Experimental

JF - Metabolism: Clinical and Experimental

IS - 11

ER -

Fluoride stimulates [³H]thymidine incorporation and alkaline phosphatase production by human osteoblasts

Abstract

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