Fluorometric Determination of DNA Nanostructure Biostability

Hannah Talbot; Bharath Raj Madhanagopal; Andrew Hayden; Ken Halvorsen; Arun Richard Chandrasekaran

doi:10.1021/acsabm.3c00287

Fluorometric Determination of DNA Nanostructure Biostability

Hannah Talbot
, Bharath Raj Madhanagopal
, Andrew Hayden
, Ken Halvorsen
, Arun Richard Chandrasekaran

University at Albany

SUNY Albany

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The analysis and improvement of DNA nanostructure biostability is one of the keys areas of progress needed in DNA nanotechnology applications. Here, we present a plate-compatible fluorometric assay for measuring DNA nanostructure biostability using the common intercalator ethidium bromide. We demonstrate the assay by testing the biostability of duplex DNA, a double crossover DNA motif, and a DNA origami nanostructure against different nucleases and in fetal bovine serum. This method scales well to measure a large number of samples using a plate reader and can complement existing methods for assessing and developing robust DNA nanostructures.

Original language	English
Pages (from-to)	3074-3078
Number of pages	5
Journal	ACS Applied Bio Materials
Volume	6
Issue number	8
DOIs	https://doi.org/10.1021/acsabm.3c00287
State	Published - Aug 21 2023

Keywords

DNA nanostructures
DNA nanotechnology
DNA origami
biostability
nuclease degradation

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title = "Fluorometric Determination of DNA Nanostructure Biostability",

abstract = "The analysis and improvement of DNA nanostructure biostability is one of the keys areas of progress needed in DNA nanotechnology applications. Here, we present a plate-compatible fluorometric assay for measuring DNA nanostructure biostability using the common intercalator ethidium bromide. We demonstrate the assay by testing the biostability of duplex DNA, a double crossover DNA motif, and a DNA origami nanostructure against different nucleases and in fetal bovine serum. This method scales well to measure a large number of samples using a plate reader and can complement existing methods for assessing and developing robust DNA nanostructures.",

keywords = "DNA nanostructures, DNA nanotechnology, DNA origami, biostability, nuclease degradation",

author = "Hannah Talbot and Madhanagopal, \{Bharath Raj\} and Andrew Hayden and Ken Halvorsen and Chandrasekaran, \{Arun Richard\}",

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year = "2023",

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doi = "10.1021/acsabm.3c00287",

language = "English",

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T1 - Fluorometric Determination of DNA Nanostructure Biostability

AU - Talbot, Hannah

AU - Madhanagopal, Bharath Raj

AU - Hayden, Andrew

AU - Halvorsen, Ken

AU - Chandrasekaran, Arun Richard

PY - 2023/8/21

Y1 - 2023/8/21

N2 - The analysis and improvement of DNA nanostructure biostability is one of the keys areas of progress needed in DNA nanotechnology applications. Here, we present a plate-compatible fluorometric assay for measuring DNA nanostructure biostability using the common intercalator ethidium bromide. We demonstrate the assay by testing the biostability of duplex DNA, a double crossover DNA motif, and a DNA origami nanostructure against different nucleases and in fetal bovine serum. This method scales well to measure a large number of samples using a plate reader and can complement existing methods for assessing and developing robust DNA nanostructures.

AB - The analysis and improvement of DNA nanostructure biostability is one of the keys areas of progress needed in DNA nanotechnology applications. Here, we present a plate-compatible fluorometric assay for measuring DNA nanostructure biostability using the common intercalator ethidium bromide. We demonstrate the assay by testing the biostability of duplex DNA, a double crossover DNA motif, and a DNA origami nanostructure against different nucleases and in fetal bovine serum. This method scales well to measure a large number of samples using a plate reader and can complement existing methods for assessing and developing robust DNA nanostructures.

KW - DNA nanostructures

KW - DNA nanotechnology

KW - DNA origami

KW - biostability

KW - nuclease degradation

UR - https://www.scopus.com/pages/publications/85163361538

U2 - 10.1021/acsabm.3c00287

DO - 10.1021/acsabm.3c00287

M3 - Article

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SN - 2576-6422

VL - 6

SP - 3074

EP - 3078

JO - ACS Applied Bio Materials

JF - ACS Applied Bio Materials

IS - 8

ER -

Fluorometric Determination of DNA Nanostructure Biostability

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