Functional cloning of BUD5, a CDC25-related gene from S. cerevisiae that can suppress a dominant-negative RAS2 mutant

Scott Powers; Enrique Gonzales; Thorkil Christensen; Jeremy Cubert; Daniel Broek

doi:10.1016/0092-8674(91)90017-S

Functional cloning of BUD5, a CDC25-related gene from S. cerevisiae that can suppress a dominant-negative RAS2 mutant

Scott Powers
, Enrique Gonzales
, Thorkil Christensen
, Jeremy Cubert
, Daniel Broek

Pathology

Stony Brook University

University of Southern California
Rutgers - The State University of New Jersey, New Brunswick

Research output: Contribution to journal › Article › peer-review

89 Scopus citations

Abstract

By searching for genes that behave like CDC25 of S. cerevisiae in their ability to counteract a dominant-negative RAS2 mutant in a wild-type RAS-dependent manner, we have isolated a CDC25-like homolog, BUD5. BUD5 is tightly linked to the MAT locus. Although overexpressed BUD5 cannot substitute for CDC25 function, we present evidence that its gene product can bind to the guanine nucleotide binding-deficient RAS2^val19ala22 gene product and thereby counteract its dominant-negative effect. We propose that BUD5 is a member of a family of CDC25-related genes that encode activators of RAS and RAS-like proteins.

Original language	English
Pages (from-to)	1225-1231
Number of pages	7
Journal	Cell
Volume	65
Issue number	7
DOIs	https://doi.org/10.1016/0092-8674(91)90017-S
State	Published - Jun 28 1991

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title = "Functional cloning of BUD5, a CDC25-related gene from S. cerevisiae that can suppress a dominant-negative RAS2 mutant",

abstract = "By searching for genes that behave like CDC25 of S. cerevisiae in their ability to counteract a dominant-negative RAS2 mutant in a wild-type RAS-dependent manner, we have isolated a CDC25-like homolog, BUD5. BUD5 is tightly linked to the MAT locus. Although overexpressed BUD5 cannot substitute for CDC25 function, we present evidence that its gene product can bind to the guanine nucleotide binding-deficient RAS2val19ala22 gene product and thereby counteract its dominant-negative effect. We propose that BUD5 is a member of a family of CDC25-related genes that encode activators of RAS and RAS-like proteins.",

author = "Scott Powers and Enrique Gonzales and Thorkil Christensen and Jeremy Cubert and Daniel Broek",

year = "1991",

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T1 - Functional cloning of BUD5, a CDC25-related gene from S. cerevisiae that can suppress a dominant-negative RAS2 mutant

AU - Powers, Scott

AU - Gonzales, Enrique

AU - Christensen, Thorkil

AU - Cubert, Jeremy

AU - Broek, Daniel

PY - 1991/6/28

Y1 - 1991/6/28

N2 - By searching for genes that behave like CDC25 of S. cerevisiae in their ability to counteract a dominant-negative RAS2 mutant in a wild-type RAS-dependent manner, we have isolated a CDC25-like homolog, BUD5. BUD5 is tightly linked to the MAT locus. Although overexpressed BUD5 cannot substitute for CDC25 function, we present evidence that its gene product can bind to the guanine nucleotide binding-deficient RAS2val19ala22 gene product and thereby counteract its dominant-negative effect. We propose that BUD5 is a member of a family of CDC25-related genes that encode activators of RAS and RAS-like proteins.

AB - By searching for genes that behave like CDC25 of S. cerevisiae in their ability to counteract a dominant-negative RAS2 mutant in a wild-type RAS-dependent manner, we have isolated a CDC25-like homolog, BUD5. BUD5 is tightly linked to the MAT locus. Although overexpressed BUD5 cannot substitute for CDC25 function, we present evidence that its gene product can bind to the guanine nucleotide binding-deficient RAS2val19ala22 gene product and thereby counteract its dominant-negative effect. We propose that BUD5 is a member of a family of CDC25-related genes that encode activators of RAS and RAS-like proteins.

UR - https://www.scopus.com/pages/publications/0025733534

U2 - 10.1016/0092-8674(91)90017-S

DO - 10.1016/0092-8674(91)90017-S

M3 - Article

C2 - 1905982

SN - 0092-8674

VL - 65

SP - 1225

EP - 1231

JO - Cell

JF - Cell

IS - 7

ER -

Functional cloning of BUD5, a CDC25-related gene from S. cerevisiae that can suppress a dominant-negative RAS2 mutant

Abstract

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