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Generation of Membrane-Derived Nanovesicles by Nitrogen Cavitation for Drug Targeting Delivery and Immunization

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

4 Scopus citations

Abstract

Extracellular vesicles (EVs) derived from cell membranes act as therapeutics and targeted drug carriers. However, the production scalability and reproducibility of EVs limit their biomedical applications. In the past few years, our lab has developed a nitrogen cavitation approach to efficiently produce EVs from any types of eukaryotic cells and bacteria. We have demonstrated that EVs derived from differentiated HL-60 cells can improve the treatment of inflammatory diseases. In addition, we have showed an increased survival of animals from bacterial infections by Pseudomonas aeruginosa after the mice were immunized with the nanovesicles derived from Pseudomonas aeruginosa membrane.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages575-589
Number of pages15
DOIs
StatePublished - 2022

Publication series

NameMethods in Molecular Biology
Volume2394

Keywords

  • Bacteria
  • Bacterium membrane
  • Cell membrane
  • Centrifugation
  • Drug delivery
  • Nanovesicles
  • Nitrogen cavitation

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