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Highly sensitive single photon detection system for multi-lane DNA sequencer

  • Dmitri Gavrilov
  • , Boris Gorbovitski
  • , Georgiy Gudkov
  • , Andrey Stepukhovich
  • , Marina Tcherevishnik
  • , Georgiy Tyshko
  • , Andriy Tsupryk
  • , Vera Gorfinkel
  • Stony Brook University

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

2 Scopus citations

Abstract

A multichannel fluorescence detection system for multi-lane DNA sequencer based on single photon counting is presented. The problem of elimination of the lane cross-talk caused by both optical and electronic cross-talk between the detection lanes is discussed. A novel method for elimination of the channel cross-talk is proposed. The calibration procedure for a DNA sequencer with lane cross-talk cancellation is described. Improvement in sensitivity of a DNA sequencer setup by introducing wide collection angle optical system is discussed.

Original languageEnglish
Title of host publicationAdvanced Photon Counting Techniques
DOIs
StatePublished - 2006
EventAdvanced Photon Counting Techniques - Boston, MA, United States
Duration: Oct 1 2006Oct 3 2006

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume6372

Conference

ConferenceAdvanced Photon Counting Techniques
Country/TerritoryUnited States
CityBoston, MA
Period10/1/0610/3/06

Keywords

  • Cross-talk cancellation
  • DNA sequencing
  • Multichannel fluorescence detector
  • Single photon counting

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