Identification and disruption of a rhoptry-localized homologue of sodium hydrogen exchangers in Toxoplasma gondii

Ariela O. Karasov; John C. Boothroyd; Gustavo Arrizabalaga

doi:10.1016/j.ijpara.2004.11.015

Identification and disruption of a rhoptry-localized homologue of sodium hydrogen exchangers in Toxoplasma gondii

Ariela O. Karasov
, John C. Boothroyd
, Gustavo Arrizabalaga

Microbiology and Immunology

University at Buffalo

Stanford University

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

Na⁺/H⁺ exchangers (NHEs) are ubiquitous membrane proteins that catalyze the exchange of Na⁺ for H⁺ and are critical in pH and cell volume regulation, as well as osmotolerance. In this study, we identify and characterize a novel NHE, TgNHE2, in Toxoplasma gondii. Immunofluorescence studies show that TgNHE2 is localized to the rhoptries, secretory organelles involved in invasion. TgNHE2 is the first intracellular NHE to be characterized in a protozoan parasite and its localization suggests possible roles for the rhoptries in osmotolerance and/or as secretory lysosomes-like granules.

Original language	English
Pages (from-to)	285-291
Number of pages	7
Journal	International Journal for Parasitology
Volume	35
Issue number	3
DOIs	https://doi.org/10.1016/j.ijpara.2004.11.015
State	Published - Mar 2005

Keywords

Osmoregulation
Rhoptry
Sodium hydrogen exchanger
Toxoplasma

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10.1016/j.ijpara.2004.11.015

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title = "Identification and disruption of a rhoptry-localized homologue of sodium hydrogen exchangers in Toxoplasma gondii",

abstract = "Na+/H+ exchangers (NHEs) are ubiquitous membrane proteins that catalyze the exchange of Na+ for H+ and are critical in pH and cell volume regulation, as well as osmotolerance. In this study, we identify and characterize a novel NHE, TgNHE2, in Toxoplasma gondii. Immunofluorescence studies show that TgNHE2 is localized to the rhoptries, secretory organelles involved in invasion. TgNHE2 is the first intracellular NHE to be characterized in a protozoan parasite and its localization suggests possible roles for the rhoptries in osmotolerance and/or as secretory lysosomes-like granules.",

keywords = "Osmoregulation, Rhoptry, Sodium hydrogen exchanger, Toxoplasma",

author = "Karasov, \{Ariela O.\} and Boothroyd, \{John C.\} and Gustavo Arrizabalaga",

year = "2005",

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AU - Karasov, Ariela O.

AU - Boothroyd, John C.

AU - Arrizabalaga, Gustavo

PY - 2005/3

Y1 - 2005/3

N2 - Na+/H+ exchangers (NHEs) are ubiquitous membrane proteins that catalyze the exchange of Na+ for H+ and are critical in pH and cell volume regulation, as well as osmotolerance. In this study, we identify and characterize a novel NHE, TgNHE2, in Toxoplasma gondii. Immunofluorescence studies show that TgNHE2 is localized to the rhoptries, secretory organelles involved in invasion. TgNHE2 is the first intracellular NHE to be characterized in a protozoan parasite and its localization suggests possible roles for the rhoptries in osmotolerance and/or as secretory lysosomes-like granules.

AB - Na+/H+ exchangers (NHEs) are ubiquitous membrane proteins that catalyze the exchange of Na+ for H+ and are critical in pH and cell volume regulation, as well as osmotolerance. In this study, we identify and characterize a novel NHE, TgNHE2, in Toxoplasma gondii. Immunofluorescence studies show that TgNHE2 is localized to the rhoptries, secretory organelles involved in invasion. TgNHE2 is the first intracellular NHE to be characterized in a protozoan parasite and its localization suggests possible roles for the rhoptries in osmotolerance and/or as secretory lysosomes-like granules.

KW - Osmoregulation

KW - Rhoptry

KW - Sodium hydrogen exchanger

KW - Toxoplasma

UR - https://www.scopus.com/pages/publications/13844289333

U2 - 10.1016/j.ijpara.2004.11.015

DO - 10.1016/j.ijpara.2004.11.015

M3 - Article

C2 - 15722080

SN - 0020-7519

VL - 35

SP - 285

EP - 291

JO - International Journal for Parasitology

JF - International Journal for Parasitology

IS - 3

ER -

Identification and disruption of a rhoptry-localized homologue of sodium hydrogen exchangers in Toxoplasma gondii

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Keywords

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