In vivo labeling of serotonin-containing neurons by 5,7-dihydroxytryptamine in Aplysia

Intrahemocoelial administration of 5,7-dihydroxytryptamine (5,7-DHT) to Aplysia californica induces a transient (< 4 h) behavioral alteration. About 5 weeks after 5,7-DHT treatment, 5-hydroxytryptamine (5-HT)-containing neurons develop dark brown pigmentation. These labeled 5-HT neurons have normal physiological and pharmacological properties when investigated electrophysiologically. This contrasts with the long-term neurotoxic effect of 5,7-DHT on vertebrate neurons. This technique will greatly facilitate visual identification of 5-HT-containing neurons and study of their physiology and actions.