Interaction of ACE2 and integrin β1 in failing human heart

Qishan Lin; Rebecca S. Keller; Barbara Weaver; Lawrence S. Zisman

doi:10.1016/j.bbadis.2004.05.005

Interaction of ACE2 and integrin β1 in failing human heart

Qishan Lin
, Rebecca S. Keller
, Barbara Weaver
, Lawrence S. Zisman

University at Albany Research Foundation

University at Albany

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

ACE2 purified from failing human heart was found to form a complex with integrin β1 by immunoprecipitation, Western blotting, activity assay, and ESI tandem mass spectroscopy. The ACE2/integrin complex showed a K_m of 6.8 μM and a V_max of 2.13 pmol/min/μl purified enzyme. Activity was optimal at pH 7.5 with Ang II substrate.

Original language	English
Pages (from-to)	175-178
Number of pages	4
Journal	Biochimica et Biophysica Acta - Molecular Basis of Disease
Volume	1689
Issue number	3
DOIs	https://doi.org/10.1016/j.bbadis.2004.05.005
State	Published - Aug 4 2004

Keywords

Angiotensin-(1-7)
Angiotensin-converting enzyme 2
Cardiomyopathy
Heart Failure
Integrin β1

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10.1016/j.bbadis.2004.05.005

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title = "Interaction of ACE2 and integrin β1 in failing human heart",

abstract = "ACE2 purified from failing human heart was found to form a complex with integrin β1 by immunoprecipitation, Western blotting, activity assay, and ESI tandem mass spectroscopy. The ACE2/integrin complex showed a Km of 6.8 μM and a Vmax of 2.13 pmol/min/μl purified enzyme. Activity was optimal at pH 7.5 with Ang II substrate.",

keywords = "Angiotensin-(1-7), Angiotensin-converting enzyme 2, Cardiomyopathy, Heart Failure, Integrin β1",

author = "Qishan Lin and Keller, \{Rebecca S.\} and Barbara Weaver and Zisman, \{Lawrence S.\}",

year = "2004",

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journal = "Biochimica et Biophysica Acta - Molecular Basis of Disease",

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AU - Lin, Qishan

AU - Keller, Rebecca S.

AU - Weaver, Barbara

AU - Zisman, Lawrence S.

PY - 2004/8/4

Y1 - 2004/8/4

N2 - ACE2 purified from failing human heart was found to form a complex with integrin β1 by immunoprecipitation, Western blotting, activity assay, and ESI tandem mass spectroscopy. The ACE2/integrin complex showed a Km of 6.8 μM and a Vmax of 2.13 pmol/min/μl purified enzyme. Activity was optimal at pH 7.5 with Ang II substrate.

AB - ACE2 purified from failing human heart was found to form a complex with integrin β1 by immunoprecipitation, Western blotting, activity assay, and ESI tandem mass spectroscopy. The ACE2/integrin complex showed a Km of 6.8 μM and a Vmax of 2.13 pmol/min/μl purified enzyme. Activity was optimal at pH 7.5 with Ang II substrate.

KW - Angiotensin-(1-7)

KW - Angiotensin-converting enzyme 2

KW - Cardiomyopathy

KW - Heart Failure

KW - Integrin β1

UR - https://www.scopus.com/pages/publications/3242769868

U2 - 10.1016/j.bbadis.2004.05.005

DO - 10.1016/j.bbadis.2004.05.005

M3 - Article

C2 - 15276642

SN - 0925-4439

VL - 1689

SP - 175

EP - 178

JO - Biochimica et Biophysica Acta - Molecular Basis of Disease

JF - Biochimica et Biophysica Acta - Molecular Basis of Disease

IS - 3

ER -

Interaction of ACE2 and integrin β1 in failing human heart

Abstract

Keywords

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