Investigation of zinc chelation in zinc-finger arrays by electrospray mass spectrometry

D. Fabris; Y. Hathout; C. Fenselau

doi:10.1021/ic9809963

Investigation of zinc chelation in zinc-finger arrays by electrospray mass spectrometry

D. Fabris
, Y. Hathout
, C. Fenselau

Pharmaceutical Sciences

Binghamton University

University of Maryland, Baltimore County
University of Maryland, College Park

Research output: Contribution to journal › Article › peer-review

52 Scopus citations

Abstract

The chelation of zinc by consensus zinc-finger arrays of the CCCC, CCHC, and CCHH type has been investigated by electrospray ionization mass spectrometry. Accurate mass measurements of the most abundant isotopic species have demonstrated that two protons are lost for each Zn(II) ion chelated. Methylation of zinc-finger peptides has revealed that two thiolate anions from cysteine side-chains are necessary to maintain chelation. The other cysteine(s) retain the thiol proton(s) and can be methylated without loss of chelating ability.

Original language	English
Pages (from-to)	1322-1325
Number of pages	4
Journal	Inorganic Chemistry
Volume	38
Issue number	6
DOIs	https://doi.org/10.1021/ic9809963
State	Published - 1999

Access to Document

10.1021/ic9809963

Cite this

@article{bd46e87cb99749e8bf9cc121e8317a27,

title = "Investigation of zinc chelation in zinc-finger arrays by electrospray mass spectrometry",

abstract = "The chelation of zinc by consensus zinc-finger arrays of the CCCC, CCHC, and CCHH type has been investigated by electrospray ionization mass spectrometry. Accurate mass measurements of the most abundant isotopic species have demonstrated that two protons are lost for each Zn(II) ion chelated. Methylation of zinc-finger peptides has revealed that two thiolate anions from cysteine side-chains are necessary to maintain chelation. The other cysteine(s) retain the thiol proton(s) and can be methylated without loss of chelating ability.",

author = "D. Fabris and Y. Hathout and C. Fenselau",

year = "1999",

doi = "10.1021/ic9809963",

language = "English",

volume = "38",

pages = "1322--1325",

journal = "Inorganic Chemistry",

issn = "0020-1669",

number = "6",

}

TY - JOUR

T1 - Investigation of zinc chelation in zinc-finger arrays by electrospray mass spectrometry

AU - Fabris, D.

AU - Hathout, Y.

AU - Fenselau, C.

PY - 1999

Y1 - 1999

N2 - The chelation of zinc by consensus zinc-finger arrays of the CCCC, CCHC, and CCHH type has been investigated by electrospray ionization mass spectrometry. Accurate mass measurements of the most abundant isotopic species have demonstrated that two protons are lost for each Zn(II) ion chelated. Methylation of zinc-finger peptides has revealed that two thiolate anions from cysteine side-chains are necessary to maintain chelation. The other cysteine(s) retain the thiol proton(s) and can be methylated without loss of chelating ability.

AB - The chelation of zinc by consensus zinc-finger arrays of the CCCC, CCHC, and CCHH type has been investigated by electrospray ionization mass spectrometry. Accurate mass measurements of the most abundant isotopic species have demonstrated that two protons are lost for each Zn(II) ion chelated. Methylation of zinc-finger peptides has revealed that two thiolate anions from cysteine side-chains are necessary to maintain chelation. The other cysteine(s) retain the thiol proton(s) and can be methylated without loss of chelating ability.

UR - https://www.scopus.com/pages/publications/0001403961

U2 - 10.1021/ic9809963

DO - 10.1021/ic9809963

M3 - Article

SN - 0020-1669

VL - 38

SP - 1322

EP - 1325

JO - Inorganic Chemistry

JF - Inorganic Chemistry

IS - 6

ER -

Investigation of zinc chelation in zinc-finger arrays by electrospray mass spectrometry

Abstract

Access to Document

Other files and links

Fingerprint

Cite this