TY - GEN
T1 - Laser Doppler speckle contrast imaging of cerebral blood flow during functional activation in rat somatosensory cortex
AU - Luo, Zhongchi
AU - Benveniste, Helene
AU - Yu, Mei
AU - Pan, Yingtian
AU - Du, Congwu
PY - 2007
Y1 - 2007
N2 - A Laser Doppler speckle contrast imaging (LDSCI) system has been developed for detecting the local cerebral blood flow (LCBF) changes in a living brain with a high temporal (subsecond) and spatial (micrometer) resolution. Near-infrared laser was used to allow the light to penetrate into the cortex beneath the brain surface and the changes in microscopic blood flow were detected due to the laser Doppler effects of moving particles (i.e., Red blood cells) on the speckle change of the imaging. To examine the sensitivity of our LDSCI system, in vivo animal study was performed to investigate activation-flow coupling in somatosensory cortex in response to electrical stimulation of hindpaw in the living rat. The functional hyperemia obtained in this study was in agreement with results from traditional point measurement using Laser Doppler flowmetry. However, the tempo-spatial feature of the LDSCI system would facilitate a better understanding of the mechanism of blood flow-neuronal activity coupling.
AB - A Laser Doppler speckle contrast imaging (LDSCI) system has been developed for detecting the local cerebral blood flow (LCBF) changes in a living brain with a high temporal (subsecond) and spatial (micrometer) resolution. Near-infrared laser was used to allow the light to penetrate into the cortex beneath the brain surface and the changes in microscopic blood flow were detected due to the laser Doppler effects of moving particles (i.e., Red blood cells) on the speckle change of the imaging. To examine the sensitivity of our LDSCI system, in vivo animal study was performed to investigate activation-flow coupling in somatosensory cortex in response to electrical stimulation of hindpaw in the living rat. The functional hyperemia obtained in this study was in agreement with results from traditional point measurement using Laser Doppler flowmetry. However, the tempo-spatial feature of the LDSCI system would facilitate a better understanding of the mechanism of blood flow-neuronal activity coupling.
KW - Activation flow coupling
KW - Laser Doppler Speckle Contrast Imaging (LDSI)
KW - Local cerebral blood flow (LCBF)
KW - Somatosensory cortex
UR - https://www.scopus.com/pages/publications/48749114529
U2 - 10.1109/NEBC.2007.4413283
DO - 10.1109/NEBC.2007.4413283
M3 - Conference contribution
SN - 1424410339
SN - 9781424410330
T3 - Proceedings of the IEEE Annual Northeast Bioengineering Conference, NEBEC
SP - 69
EP - 70
BT - 33rd Annual Northeast Bioengineering Conference - Engineering Innovations in Life Sciences and Healthcare, NEBC
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 33rd Annual Northeast Bioengineering Conference, NEBC
Y2 - 10 March 2007 through 11 March 2007
ER -