Levels of adrenodoxin, NADPH-cytochrome P-450 reductase and cytochromes P-45011β, P-45021, P-450scc, in adrenal zona fasciculata-reticularis tissue from androgen-treated rats

Alexander C. Brownie; C. Ramana Bhasker; Michael R. Waterman

doi:10.1016/0303-7207(88)90086-X

Levels of adrenodoxin, NADPH-cytochrome P-450 reductase and cytochromes P-450_11β, P-450₂₁, P-450_scc, in adrenal zona fasciculata-reticularis tissue from androgen-treated rats

Alexander C. Brownie
, C. Ramana Bhasker
, Michael R. Waterman

Biochemistry

University at Buffalo

University of Texas Southwestern Medical Center

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Treatment of rats with methylandrostenediol (MAD), an anabolic androgen, caused a profound reduction (65%) in the level of cytochrome P-450_11β in rat adrenocortical mitochondria as measured by immunoblots using a specific antibody. The decreases in mitochondrial cytochrome P-450_scc (15%) and adrenodoxin (20%) were much less than that observed for cytochrome P-450_11β. A 35% decrease in adrenal microsomal cytochrome P-450₂₁ and NADPH-cytochrome P-450 reductase levels was brought about by the treatment with MAD. The data establish that the preferential decrease in adrenal steroid 11β-hydroxylase activity associated with androgen treatment results from a decrease in cytochrome P-450_11β. This is consistent with the role of 11-deoxycorticosterone in the pathogenesis of androgen-induced hypertension in rats.

Original language	English
Pages (from-to)	15-20
Number of pages	6
Journal	Molecular and Cellular Endocrinology
Volume	55
Issue number	1
DOIs	https://doi.org/10.1016/0303-7207(88)90086-X
State	Published - Jan 1988

Keywords

Adrenal cortex
Methylandrostenediol
Steroid hydroxylase

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10.1016/0303-7207(88)90086-X

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@article{d89b1d6b8de7457685e1b9deb00cfb5e,

title = "Levels of adrenodoxin, NADPH-cytochrome P-450 reductase and cytochromes P-45011β, P-45021, P-450scc, in adrenal zona fasciculata-reticularis tissue from androgen-treated rats",

abstract = "Treatment of rats with methylandrostenediol (MAD), an anabolic androgen, caused a profound reduction (65\%) in the level of cytochrome P-45011β in rat adrenocortical mitochondria as measured by immunoblots using a specific antibody. The decreases in mitochondrial cytochrome P-450scc (15\%) and adrenodoxin (20\%) were much less than that observed for cytochrome P-45011β. A 35\% decrease in adrenal microsomal cytochrome P-45021 and NADPH-cytochrome P-450 reductase levels was brought about by the treatment with MAD. The data establish that the preferential decrease in adrenal steroid 11β-hydroxylase activity associated with androgen treatment results from a decrease in cytochrome P-45011β. This is consistent with the role of 11-deoxycorticosterone in the pathogenesis of androgen-induced hypertension in rats.",

keywords = "Adrenal cortex, Methylandrostenediol, Steroid hydroxylase",

author = "Brownie, \{Alexander C.\} and Bhasker, \{C. Ramana\} and Waterman, \{Michael R.\}",

year = "1988",

month = jan,

doi = "10.1016/0303-7207(88)90086-X",

language = "English",

volume = "55",

pages = "15--20",

journal = "Molecular and Cellular Endocrinology",

issn = "0303-7207",

publisher = "Elsevier Ireland Ltd",

number = "1",

}

Levels of adrenodoxin, NADPH-cytochrome P-450 reductase and cytochromes P-450_11β, P-450₂₁, P-450_scc, in adrenal zona fasciculata-reticularis tissue from androgen-treated rats. / Brownie, Alexander C.; Bhasker, C. Ramana; Waterman, Michael R.
In: Molecular and Cellular Endocrinology, Vol. 55, No. 1, 01.1988, p. 15-20.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Levels of adrenodoxin, NADPH-cytochrome P-450 reductase and cytochromes P-45011β, P-45021, P-450scc, in adrenal zona fasciculata-reticularis tissue from androgen-treated rats

AU - Brownie, Alexander C.

AU - Bhasker, C. Ramana

AU - Waterman, Michael R.

PY - 1988/1

Y1 - 1988/1

N2 - Treatment of rats with methylandrostenediol (MAD), an anabolic androgen, caused a profound reduction (65%) in the level of cytochrome P-45011β in rat adrenocortical mitochondria as measured by immunoblots using a specific antibody. The decreases in mitochondrial cytochrome P-450scc (15%) and adrenodoxin (20%) were much less than that observed for cytochrome P-45011β. A 35% decrease in adrenal microsomal cytochrome P-45021 and NADPH-cytochrome P-450 reductase levels was brought about by the treatment with MAD. The data establish that the preferential decrease in adrenal steroid 11β-hydroxylase activity associated with androgen treatment results from a decrease in cytochrome P-45011β. This is consistent with the role of 11-deoxycorticosterone in the pathogenesis of androgen-induced hypertension in rats.

AB - Treatment of rats with methylandrostenediol (MAD), an anabolic androgen, caused a profound reduction (65%) in the level of cytochrome P-45011β in rat adrenocortical mitochondria as measured by immunoblots using a specific antibody. The decreases in mitochondrial cytochrome P-450scc (15%) and adrenodoxin (20%) were much less than that observed for cytochrome P-45011β. A 35% decrease in adrenal microsomal cytochrome P-45021 and NADPH-cytochrome P-450 reductase levels was brought about by the treatment with MAD. The data establish that the preferential decrease in adrenal steroid 11β-hydroxylase activity associated with androgen treatment results from a decrease in cytochrome P-45011β. This is consistent with the role of 11-deoxycorticosterone in the pathogenesis of androgen-induced hypertension in rats.

KW - Adrenal cortex

KW - Methylandrostenediol

KW - Steroid hydroxylase

UR - https://www.scopus.com/pages/publications/0023829734

U2 - 10.1016/0303-7207(88)90086-X

DO - 10.1016/0303-7207(88)90086-X

M3 - Article

C2 - 3129324

SN - 0303-7207

VL - 55

SP - 15

EP - 20

JO - Molecular and Cellular Endocrinology

JF - Molecular and Cellular Endocrinology

IS - 1

ER -

Levels of adrenodoxin, NADPH-cytochrome P-450 reductase and cytochromes P-450_11β, P-450₂₁, P-450_scc, in adrenal zona fasciculata-reticularis tissue from androgen-treated rats

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Keywords

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