Lifting the cryopreservation 'cap': improved survival and CPA reduction through molecular biological analysis

John M. Baust; Robert Van Buskirk; John G. Baust

Lifting the cryopreservation 'cap': improved survival and CPA reduction through molecular biological analysis

John M. Baust
, Robert Van Buskirk
, John G. Baust

Biology

Binghamton University

State University of New York Binghamton University

Research output: Contribution to journal › Conference article › peer-review

Abstract

A study was conducted focusing on 1) the shift of the CP 'cap' (CPA-dependent survival barrier) through the use of HTS as a CPA carrier and 2) the molecular activation of the apoptotic caspase cascade following CP in human fibroblast, kidney, and liver cell lines. Cells were subjected to a controlled rate freezing protocol in media or HTS containing 0-20% DMSO. Post-thaw viability was assessed using a metabolic indicator, alamarBlue, and a DNA stain, SytoDye. Caspase expression following CP was investigated by RT-PCR analysis. Results show that 1) CP results in a survival 'cap,' 2) HTS 'lifts' the CP 'cap' and allows for a reduction in DMSO, 3) gene activation occurs following CP, and 4) the control of CP-induced gene activation may increase CP effectiveness.

Original language	English
Pages (from-to)	S-124
Journal	Annals of Biomedical Engineering
Volume	28
Issue number	SUPPL. 1
State	Published - 2000
Event	2000 Annual Fall Meeting of the Biomedical Engineering Society - Washington, WA, USA Duration: Oct 12 2000 → Oct 14 2000

Cite this

@article{ec3e7ce431de4744b861e80bd263dc29,

title = "Lifting the cryopreservation 'cap': improved survival and CPA reduction through molecular biological analysis",

abstract = "A study was conducted focusing on 1) the shift of the CP 'cap' (CPA-dependent survival barrier) through the use of HTS as a CPA carrier and 2) the molecular activation of the apoptotic caspase cascade following CP in human fibroblast, kidney, and liver cell lines. Cells were subjected to a controlled rate freezing protocol in media or HTS containing 0-20\% DMSO. Post-thaw viability was assessed using a metabolic indicator, alamarBlue, and a DNA stain, SytoDye. Caspase expression following CP was investigated by RT-PCR analysis. Results show that 1) CP results in a survival 'cap,' 2) HTS 'lifts' the CP 'cap' and allows for a reduction in DMSO, 3) gene activation occurs following CP, and 4) the control of CP-induced gene activation may increase CP effectiveness.",

author = "Baust, \{John M.\} and Buskirk, \{Robert Van\} and Baust, \{John G.\}",

year = "2000",

language = "English",

volume = "28",

pages = "S--124",

journal = "Annals of Biomedical Engineering",

issn = "0090-6964",

publisher = "Springer",

number = "SUPPL. 1",

note = "2000 Annual Fall Meeting of the Biomedical Engineering Society ; Conference date: 12-10-2000 Through 14-10-2000",

}

TY - JOUR

T1 - Lifting the cryopreservation 'cap'

T2 - 2000 Annual Fall Meeting of the Biomedical Engineering Society

AU - Baust, John M.

AU - Buskirk, Robert Van

AU - Baust, John G.

PY - 2000

Y1 - 2000

N2 - A study was conducted focusing on 1) the shift of the CP 'cap' (CPA-dependent survival barrier) through the use of HTS as a CPA carrier and 2) the molecular activation of the apoptotic caspase cascade following CP in human fibroblast, kidney, and liver cell lines. Cells were subjected to a controlled rate freezing protocol in media or HTS containing 0-20% DMSO. Post-thaw viability was assessed using a metabolic indicator, alamarBlue, and a DNA stain, SytoDye. Caspase expression following CP was investigated by RT-PCR analysis. Results show that 1) CP results in a survival 'cap,' 2) HTS 'lifts' the CP 'cap' and allows for a reduction in DMSO, 3) gene activation occurs following CP, and 4) the control of CP-induced gene activation may increase CP effectiveness.

AB - A study was conducted focusing on 1) the shift of the CP 'cap' (CPA-dependent survival barrier) through the use of HTS as a CPA carrier and 2) the molecular activation of the apoptotic caspase cascade following CP in human fibroblast, kidney, and liver cell lines. Cells were subjected to a controlled rate freezing protocol in media or HTS containing 0-20% DMSO. Post-thaw viability was assessed using a metabolic indicator, alamarBlue, and a DNA stain, SytoDye. Caspase expression following CP was investigated by RT-PCR analysis. Results show that 1) CP results in a survival 'cap,' 2) HTS 'lifts' the CP 'cap' and allows for a reduction in DMSO, 3) gene activation occurs following CP, and 4) the control of CP-induced gene activation may increase CP effectiveness.

UR - https://www.scopus.com/pages/publications/18544410779

M3 - Conference article

SN - 0090-6964

VL - 28

SP - S-124

JO - Annals of Biomedical Engineering

JF - Annals of Biomedical Engineering

IS - SUPPL. 1

Y2 - 12 October 2000 through 14 October 2000

ER -

Lifting the cryopreservation 'cap': improved survival and CPA reduction through molecular biological analysis

Abstract

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