Mammalian two-hybrid assay for detecting protein-protein interactions in vivo.

Jae Woon Lee; Soo Kyung Lee

doi:10.1385/1-59259-762-9:327

Mammalian two-hybrid assay for detecting protein-protein interactions in vivo.

Biological Sciences

University at Buffalo

Research output: Contribution to journal › Review article › peer-review

18 Scopus citations

Abstract

Mammalian two-hybrid assay is a convenient, powerful tool to investigate protein-protein interactions in vivo. In particular, this method has a major advantage over the better known yeast version in that one can study interactions between mammalian proteins that may not fold correctly in yeast or that require post-translational modification or external stimulation that are not present in yeast.

Original language	English
Pages (from-to)	327-336
Number of pages	10
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	261
DOIs	https://doi.org/10.1385/1-59259-762-9:327
State	Published - 2004

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10.1385/1-59259-762-9:327

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title = "Mammalian two-hybrid assay for detecting protein-protein interactions in vivo.",

abstract = "Mammalian two-hybrid assay is a convenient, powerful tool to investigate protein-protein interactions in vivo. In particular, this method has a major advantage over the better known yeast version in that one can study interactions between mammalian proteins that may not fold correctly in yeast or that require post-translational modification or external stimulation that are not present in yeast.",

author = "Lee, \{Jae Woon\} and Lee, \{Soo Kyung\}",

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doi = "10.1385/1-59259-762-9:327",

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AU - Lee, Jae Woon

AU - Lee, Soo Kyung

PY - 2004

Y1 - 2004

N2 - Mammalian two-hybrid assay is a convenient, powerful tool to investigate protein-protein interactions in vivo. In particular, this method has a major advantage over the better known yeast version in that one can study interactions between mammalian proteins that may not fold correctly in yeast or that require post-translational modification or external stimulation that are not present in yeast.

AB - Mammalian two-hybrid assay is a convenient, powerful tool to investigate protein-protein interactions in vivo. In particular, this method has a major advantage over the better known yeast version in that one can study interactions between mammalian proteins that may not fold correctly in yeast or that require post-translational modification or external stimulation that are not present in yeast.

UR - https://www.scopus.com/pages/publications/3242671815

U2 - 10.1385/1-59259-762-9:327

DO - 10.1385/1-59259-762-9:327

M3 - Review article

C2 - 15064467

SN - 1064-3745

VL - 261

SP - 327

EP - 336

JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

ER -

Mammalian two-hybrid assay for detecting protein-protein interactions in vivo.

Abstract

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